Lead and lead compounds

cytoxicity and mutagenicity following lead acetate in CL3 human lung cancer cells. Toxicology 2008b; 250: 55-61. Wang CY, Wang YT, Tzeng DW, Yang JL. ...

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Lead and lead compounds by Hartwig Muhle PhD and Kyle Steenland PhD Citation for most recent IARC review IARC Monographs 87, 2006 Current evaluation Conclusion from the previous Monograph: Inorganic lead and lead compounds are probably carcinogenic to humans (Group 2 A). Exposure and biomonitoring The CDC has an ongoing exposure and biomonitoring program for a number of chemicals including lead (CDC, National Exposure Report, 2005) which follows changes in blood lead concentrations over time. More recently, the FLEHS Biomonitoring Study (Ketelslegers et al., 2008) has reported the results of multi-plex genotyping as a biomarker of susceptibility to carcinogenic exposures. These studies, which included lead, reported a positive relationship between lead exposure and the carcinoembryonic antigen BRCA2N372H. Lead associated levels of this antigen were also found to be 1.9 times higher in persons carrying the double variant allele (heterozygous) and 1.6 times higher in persons carrying the wild type. An even greater increase was observed in smokers in response to lead exposure. The importance of these data rests with a better understanding of the relationship between genetic inheritance, lead exposure and risk of cancer. Cancer in humans (limited, Vol 87, 2006). The 2006 evaluation was based primarily on six larger historical cohorts exposed to inorganic lead. Data on the effects of exposure to organic lead was sparse and no conclusions could be drawn. Historical organic lead exposure was largely confined to tetra-ethyl lead used as an additive in gasoline (now discontinued); tetra-ethyl lead was broken down into inorganic lead when gasoline was burned. Regarding the six cohorts exposed to inorganic lead, stomach cancer was consistently elevated (30-50%) in four of the five cohorts where stomach cancers were reported. Lung, kidney, and brain cancer showed elevation in some studies but were not consistent. Since 2006 there are five studies with new information, although none are large or would change the conclusions of the 2006 Working Group. Wijngaarden et al. (2006) studied brain cancer in the National Longitudinal Mortality Study, a prospective census-based cohort study of the United States population in 1979-1989 (n=317,968). Using a job-exposure matrix and industry and occupation from the census, the authors found increased risk of brain cancer in jobs likely to have involved lead exposure (RR 1.5, 0.9-2.3)(18% exposed), with indications of an exposure-response trend based on 12

estimated intensity of exposure. This study is limited due to lack of direct data on exposure, but adds to the positive evidence for brain cancer. Rousseau et al. (2007) conducted nested-case control studies for 11 types of cancers and estimated lead exposure, with exposure estimated based on work history and expert assessment (Montreal/Siemiatycki data base). 17% of subjects were exposed to inorganic lead, while 3% were exposed to organic lead. Stomach cancer was associated with organic lead (ORs 2.0 (1.1-3.8) and 3.0 (1.2-7.3) depending on control group), but there was no association with inorganic lead, nor were there any notable positive associations for other cancers. Again this study is limited due to lack of direct data on exposure, but the lack of association weakens the IARC 2006 conclusion. Lam et al. (2006) linked 3192 lead-exposed workers in New Jersey, identified by a NIOSHsponsored lead surveillance program, to the NJ Cancer Registry. All cohort members had measured blood leads >25 µg/Dl at some point in the past. They found 83 incidence cancer cases, with a deficit of cancer overall (0.51, 0.41-0.62). There were no notable excesses (stomach cancer SIR 1.1, 4 cases). This study is limited by small numbers, and possible under-ascertainment of cases due to out-of-state migration. Siddiqui et al. (2006) conducted a case-control of breast cancer in India, with 50 malignant and 50 benign cases. Blood lead was significantly higher in the malignant case croups, as were zinc, iron, and calcium. This study is limited as blood lead was present at background environmental levels and reflected exposure in recent past (3 months). Rajaraman et al. (2006) reported increased rates of brain tumors (gliomas and menigomas) in relation to genetic polymorphisms in the ALAD gene and lead exposure suggesting a need to take genetic inheritance of ALAD polymorphisms into account with respect to lead exposures and development of these types of cancer. The ALAD2 allele was found to increase the risk of menigioma from 1.1 to 5.6 and 12.8 based upon estimated based upon intermediate and longer term cumulative lead exposures respectively. ALAD may play an important role in the bioavailability of lead to sensitive molecular sites which is known to vary as a function of the ALAD2 allele (Scinicariello et al., 2007) and lead inhibition of this enzyme and others in the heme biosynthetic pathway may also contribute to the generation of oxidative stress via generation of reactive oxygen species. Cancer in experimental animals (sufficient, Vol 87, 2006) No new carcinogenicity assays have been reported since the last evaluation since 2002. In contrast, there have been a number of studies reporting the inhibitory effects of lead exposure on sperm development and motility in male experimental animals (Kaspercyk et al., 2008, Wang et al., 2008)


Mechanisms of carcinogenicity The mechanisms of lead induced cancers in experimental systems are complex appear to involve oxidative stress (Jurczuk et al., 2006) and may involve lead interaction with zinc finger proteins (Jarzecki et al., 2007), induction of apoptosis (Xu et al., 2006, 2008), altered cell signaling pathways (Wang et al., 2008b, 2009) and regulation of lead interactions with cellular genetic machinery by high affinity lead - binding proteins (Fowler et al., 1994, Rajaraman, 2006). Research needs and recommendations Additional epidemiologic information can be provided by large cohorts with established exposure above background environmental levels. It appears likely that there will be a mortality study of approximately 50,000 workers with past measured blood lead levels above 25 µg/Dl in the United States (NIOSH ABLES surveillance program), which should prove informative. There are also an additional 50,000 subjects with lower blood leads. Subsequent possible case-control studies of cancers of interest (stomach, brain, kidney, lung) could also provide more information. This study could be strengthened by the addition of two components: 1) measurement of a sample of subjects for bone lead to determine the correlation of the blood lead measurements with cumulative exposure as measured by bone lead, and 2) assessment of whether Helicobacter pylori infection has been more common among those with higher blood leads. If so such infection could either be a mechanism by which lead caused higher rates of stomach cancer, or it could be a confounder. In addition, given positive results from the FLEHS study (Keteleslegers et al., 2008) and the report by Rajaraman et al. (2006) it is clear that future epidemiological studies of relationships between lead exposures and cancer should include evaluation of genetic susceptibility factors if at all possible. Further experimental research studies are needed to evaluate the complex mechanisms by which lead may cause cancer with particular emphasis on the roles of oxidative stress / apoptosis and the roles of cellular defense mechanisms, signaling pathways and intracellular lead binding patterns in mediating these processes. Selected relevant publications CDC. Centers for Disease Control and Prevention. Third National Report on Human Exposure to Environmental Chemicals. Atlanta (GA): CDC, 2005. Fowler BA, Kahng, MW, Smith DR. Role of lead binding proteins in renal cancer. Environ Health Perspect 1994; 102: 115-116. Jarzecki AA. Lead-poisoned zinc fingers: quantum mechanical exploration of structure, coordination, and electronic excitations. Inorg Chem 2007; 46: 7509-7521.


Jurczuk M, Moniuszko-Jakoniuk J, Brzóska MM. Involvement of some low-molecular thiols in the peroxidative mechanisms of lead and ethanol action on rat liver and kidney. Toxicology 2006; 219: 11-21. Kasperczyk A, Kasperczyk S, Horak S, et al. Assessment of semen function and lipid peroxidation among lead exposed men. Toxicol Appl Pharmacol 2008; 228: 378-384. Ketelslegers HB, Gottschalk WH, Koppen G, et al. Multiplex genotyping as a biomarker for susceptibility to carcinogenic exposure in the FLEHS biomonitoring study. Cancer Epidemiol Biomark Prev 2008; 17: 1902-1912. Rajaraman P, Stewart PA, Samet JM, et al. Lead, genetic susceptibility, and risk of adult brain tumors. Cancer Epidemiol Biomark Prev 2006; 15: 2514-2520. Rousseau MC, Parent ME, Nadon L, Latreille B, Siemiatycki J. Occupational exposure to lead compounds and risk of cancer among men: a population-based case-control study. Am J Epidemiol 2007; 166: 1005-1014. Scinicariello F, Murray HE, Moffett DB, et al. Lead and δ-aminolevulinic acid dehydratase polymorphism: where does it lead? A meta-analysis. Environ Health Perspect 2007; 115: 3541. Siddiqui MK, Jyoti, Singh S, Mehrotra PK, Singh K, Sarangi R. Comparison of some trace elements concentration in blood, tumor free breast and tumor tissues of women with benign and malignant breast lesions: an Indian study. Environ Int 2006; 32: 630-637. van Wijngaarden E, Dosemeci M. Brain cancer mortality and potential occupational exposure to lead: findings from the National Longitudinal Mortality Study, 1979-1989. Int J Cancer 2006; 119: 1136-1144. Wang L, Xun P, Zhao Y, et al. Effects of lead exposure on sperm concentrations and testes weight in male rats: a meta-regression analysis. J Toxicol Environ Health A 2008a; 71: 454463. Wang CY, Lin YW, Yang JL. Activation of protein kinase Calpha signaling prevents cytoxicity and mutagenicity following lead acetate in CL3 human lung cancer cells. Toxicology 2008b; 250: 55-61. Wang CY, Wang YT, Tzeng DW, Yang JL. Lead acetate induces EGFR activation upstream of SFK and PKCalpha linkage to the Ras/Raf-1/ERK signaling. Toxicol Appl Pharmacol 2009; 235: 244-252.


Xu J, Ji LD, Xu LH. Lead-induced apoptosis in PC 12 cells: involvement of p53, Bcl-2 family and caspase-3. Toxicol Lett 2006; 166: 160-167. Xu J, Lian LJ, Wu C, et al. Lead induces oxidative stress, DNA damage and alteration of p53, Bax and Bcl-2 expressions in mice. Food and Chem Toxicol 2008; 46: 1488-1494.

Indium phosphide and other indium compounds includes indium phosphide, indium arsenide, indium tin oxide, CIS, CIGS by Bruce A. Fowler PhD, Mary Schubauer-Berigan PhD, and Cynthia J. Hines MS.

Citation for most recent IARC review IARC Monographs 86, 2006 Current evaluation Conclusion from the previous Monograph: Indium phosphide is probably carcinogenic to humans (Group 2A). Despite a lack of evidence from human studies, the carcinogenicity was upgraded because of the “extraordinarily high incidences of malignant neoplasms of the lung in male and female rats and mice; increased incidences of pheochromocytomas in male and female rats; and increased incidences of hepatocellular neoplasms in male and female mice.” These occurred at very low doses and short exposure periods. Exposure and biomonitoring Occupational exposure Since the publication of Monograph 86, production of indium compounds has increased but appears limited by the low rate of refining indium as a byproduct of zinc and lead-zinc smelting (Hageluken 2006). The most well documented exposed population likely remains the semiconductor industry. Chen (2007) estimates that over 30,000 people were employed in 2007 among 350 semiconductor manufacturing firms in Taiwan. In the United States, 255,000 workers were estimated to be involved in the manufacture or application of semiconductor chips in 2004, possibly including exposure to inorganic indium compounds (http://www.siaonline.org/cs/papers_publications/press_release_detail?pressrelease.id=221 ). [It is unlikely that all workers in the global semiconductor industry have been exposed to indium compounds and these workers may be exposed to a variety of other Group 1 or 2 human carcinogens].