Index Gas Chromatography Applications

Index Gas Chromatography Applications Content Page Chemical Biological & Industrial 316-336 Analysis of Simazin 316 ... 2- Methyl acetate 3- Ethyl ace...

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Index Gas Chromatography Applications Content

Chemical Biological & Industrial

Analysis of Simazin Solvents Trimethylsilyl Derivatives of Methylglycosides From Bacteriological Polysacarides Solvents Impurities of Methyl Pivalate Analysis of Solvents Separation of Monomers In Paints Separation of Nitrosoamines Sodium Cyclamate Impurities Phenol In Resins Separation of Solvents Chloroform Impurities Impurities of N-butanol Alcohol Impurities Separation of Solvents Impurities of Ethanol Separation Impurities of Butyl Acrylate Separation of Hydrocarbons (Fast Chromatography) Chloroform Purity Polywax 655 Impurities of P-phenetidine Drugs In Urine Amantadine Hydrochloride Impurities Ethanolamines Separation (25 Ng/peak Level) Usp Solvents Column Trb-g27+guardcolumn 5m Analysis of Cyclosiloxanes EPA 601/602 Purgeable Halocarbons Mix Plus 2-chloroethyl Vinyl Ether Solvents In Water Industrial Solvents Industrial Solvents In Raw Materials Aromatic Solvents Mixture of Solvents Glycols In Water Purity of Chloroform Impurities of Dimethylacetamide Impurities of Lactic Acid Alcohols In Blood Impurities In Solvents Pollutants In Blood Alcohols In Blood Impurities In Toluene Mixture of Solvents And Isomers of N-heptane Petrol 331 Alcohols In Blood Amines Aminoacids Hydrocarbons Volatil Solvents No Halogenated Bacterial Acid Methyl Esters Mix Residual Solvents In Dmso Common Industrial Solvents Reaction Products of 1,1-diethoxy Butane Separation of Acetic Acid And Acetic Anhydride Petrol

Environmental

Analysis of Soil And Sludge of A Water-treatment Plant Analysis of Polycyclic Aromatic Hydrocarbons Analysis of Pcb’s Separation of Btx Separation of Volatil Solvents Glycols Nitriles And Amines In Water Separation of Organochlorinated Pesticides Separation of Aldehydes Separation of Btex Isomers Chlorinated Pesticides Aldehydes In Air Sample Separation of Btex Isomers Analysis of Hydrocarbons (Gasoil) Pesticides Amines Separation of Solvents Polyaromatic Hydrocarbons Pcb´s In Mussels Dioxines And Furanes Separation TRB-5ms Dioxines TRB-5ms Furanes TRB-5ms Dioxines. Emision Sample TRB-5ms Furanes. Emision Sample TRB-5ms Dioxines - Food Sample TRB-5ms Furanes - Food Sample Optimum Resolution In Semivolatile Compounds Analysis Pesticides Analysis Purifier Sludge Analysis

Page 316-336 316 316 316 316 316 317 317 317 318 318 318-319 319 319 320 321 321 322 322 322 322 323 323 323 323 324 324 324 325 325 325-326 326 326 326 327 327 327 327 328 328-329 329 330 331 332 332 333 333 334 334 335 335 336 336 336 337-366 337 337 337 338 338 338 338 339 339 339 340-341 341 341 342 342 343 343 343 344 344 345 345 346 346 347 347 348-349 350 350

Content

Page

Separation of Pesticides Free Acids In Water Solvents In Water (100 Ppm) Analysis of Fire Residues (Pyrolisis) Analysis of Nonylphenols Analysis of Aroclors Phenols EPA 604 Analysis of Pesticides Organochlorinated Pesticides EPA 608 Separation of Phenols EPA 604 Aroclor 1254 In Isooctane (10 Ppm) Aroclor 1260 In Isooctane (10 Ppm) Superimposed Individual Aroclors 1242 (Green), 1254 (Blue), 1260 (Red) In Isooctane (10 Ppm) Mix of Aroclors 1242, 1254 And 1260 In Isooctane (10 Ppm) Aroclor 1242 In Isooctane (10 Ppm) Pcbs 358 EPA 601 Purgeable Halocarbons Mix Analysis of Pyridines Analysis of Pesticides Amines Separation of Phtalates Separation of Aromatic Hydrocarbons Separation of Solvents Phenols EPA 604 Separation of Solvents Separation of PAE (Phthalate Alkyl Ester) Mix EPA 604 Aromatic Compounds Polycyclic Aromatic Hydrocarbons (PAH EPA 610) Clp Pesticides (EPA 8081)

351-352 353 353 353 354 354 354 354 355 355 356 356

Food & Flavors

Ethyl Carbamate In Wine Tuna Oil With Added Dha Alcohols Separation of Fames Essential Oil Separation of Alcohols Analysis of Chloropicrine In Wines Separation of Volatiles In Alcoholic Beverages Separation of Acrylamide Identification of Wax In Olive Oil Separation of Acetoine In Vinegar Separation of Polyols In Wine Analysis of Rapeseed Oil (Fast Chromatography) Analysis of Rapeseed Oil Alcohols And Terpenic Acids (Olive Oil) Analysis Bergamol Aldehydes Pesticides In Wines Separation of Sterols In Olive Oil (Virgen Extra) Hydrogenated Soya Oil Separation of Fames Phenols-anisol In Wine Phenol-anisol In Wine (Fast Chromatography) Phytosanitary Analysis In Wine Separation of Sterols In Olive Oil (Orujo) Chlorinated Solvents In Olive Oil Analysis of Volatiles In Olive Oil Separation of Volatiles In Alcoholic Beverages Analysis of Glycols In Wine Analysis of Polyols In Wine Phenols EPA 604 Separation of Fames Separation of Methyl Esters (Rapessed Oil) Flavors In Wine Lavander Flavor Flavors (Lavander, Essential Oil) Analysis of Pesticides 378 Sterols Analysis (Refinated Olive Oil) Analysis of Cis-trans Isomers Analysis of Methyl Esters Separation of Sugars (As Alditol Acetates) Separation of Sterols Separation of Volatiles In Alcoholic Beverages Sterols In Olive Oil Triglycerides In Milky Fat Allergens In Cosmetics Separation of Methyl Esters (Fames) Separation of Fames Separation of Methyl Esters In Soya Oil Glycols In Wine Methanol And Higher Alcohols Pufa I Pufa II Pufa III 37 Fame Mix 37 Fame Mix- Ms Detector

358 357 357 358 359 359 360 360 361 362 363 364 364 364 365 365 366 367-391 367 367 367 368 368 369 369 369 370 370 370 370 371 371 371 371 372 372 372 373 373 374 374 374 375 375 375 376 376 376 376 377 377 377 378 378 379 379 379-380 380 380 381-383 384 385 385 386 387 387 388 388 389 389 390 390 391

315

Chemical Biological & Industrial ANALYSIS OF SIMAZIN

SOLVENTS

2

Column: TRB-50, P/N TR-500232 Dimensions: 30m x 0.25mm x 0.25 µm Injection: 0.2 µL standard (20 ng on column), split 1:10, 280ºC Carrier gas: H2, constant pressure 11 psi (75.8 KPa) Oven temperature: 200ºC (Isothermal) Detector: FID, 280ºC

Column: TRB-624, P/N TR-603035 Dimensions: 30m x 0.53mm x 3.0 µm Injection: wet needle, split 1:5, 260ºC Carrier gas: He, constant pressure 3 psi (20.7 KPa). Oven temperature: 40ºC (isothermal) Detector: FID, 260ºC

4

1 Peak Name

Peak Name

1 2

1 2 3 4 5

Simazin (2,4-Bis(ethylamino)-6-chloro-1,3,5-triazine) Dibutylphthalate (I.St.)

1

Methanol Ethanol Acetone Isopropanol Methylene chloride

2 3

5

TKG 1001

TRIMETHYLSILYL DERIVATIVES OF METHYLGLYCOSIDES FROM BACTERIOLOGICAL POLYSACARIDES

TKG 1004

IMPURITIES OF METHYL PIVALATE Column: Meta .X5, P/N TR-825035 Dimensions: 30m x 0.53mm x 5.0 µm Injection: 0.5 µL Methyl Pivalate (+ 0.1% of each impurity), split 1:25, 250ºC Carrier gas: H2, constant pressure, 2.5 psi (17.23 KPa). Oven program: 40ºC(10min) @ 5ºC/min to 130ºC Detector: FID, 280ºC

Column: TRB-1, P/N TR-110222 Dimensions: 25m x 0.25mm x 0.25 µm Injection: 1 µL, split1:3, 250ºC Carrier gas: He, constant pressure 10 psi (69 KPa). Oven program: 140ºC(2min) @ 8ºC/min to 280ºC(30min) Detector: MS, 300ºC Chromatogram provided by Antonio Gil from Department of Organic Chemestry. CSIC (Sevilla)

Peak Name

1

123456-

12345-

4

Glycerol TMS Ramnose TMS Manose β-Glucose α-Glucose Inositol

3 2

Methanol Ethanol Pinacolone Methyl Pivalate Ethyl Pivalate

5 6

1

2

3

TKG 1088 316

Peak Name

4

5

TKG 1085

Chemical Biological & Industrial ANALYSIS OF SOLVENTS

15

Column: TRB-WAX, P/N TR-142065 Dimensions: 60m x 0.53mm x 2.0 µm Injection: wet needle, split, 250ºC Carrier gas: H2, constant pressure 4 psi (27.6 KPa). Oven program: 55ºC(20min) @ 3ºC/min to 220ºC(15min) Detector: FID, 260ºC

19

17

10

18 9 11

16

Peak Name 12345678910111213141516171819-

Acetone Methyl acetate Ethyl acetate Methanol + MEK Isopropanol Ethanol MIKB Methoxypropyl acetate Isobutyl acetate Toluene Methoxypropanol n-butyl acetate Isobutanol n-butanol p,m-xylenes o-xylene Ethylglycol Diacetone alcohol Butyl glycol

7

13 8 12

4

3

5 6

1

14 2

TKG 1003

SEPARATION OF MONOMERS IN PAINTS

SEPARATION OF NITROSOAMINES

Column: Meta .WAX, P/N TR-811035 Dimensions: 30m x 0.53mm x 1.0 µm Injection: 1 µL Monomers mixture (20ppm, 100ppm toluene in DMSO), split 1:50, 240ºC Carrier gas: He, 4 psi (27.6 KPa) Oven temperature: 40ºC(5min) @ 15ºC/min to 180ºC(15min) Detector: FID, 240ºC

Column: TR-WAX DB, P/N TR-932112 Dimensions: 15m x 0.25mm x 0.20 µm Injection: 1 µL standard 100 ppm in n-hexane, split 1:10, 260ºC Carrier gas: H2, constant pressure 8 psi (55.1 KPa) Oven temperature: 50ºC(2min) @ 10ºC/min to 150ºC @ 15ºC/min to 240ºC(10min) Detector: FID, 260ºC

2

Peak Name

Peak Name

1234-

12345678910-

Methyl acrylate Toluene Butyl acrylate Styrene

1

3

4

2

4 1

3

TKG 1005

N-nitrosodimethylamine N-nitrosodiethylamine N-nitrosodiisopropylamine N-nitrosodi-n-propylamine N-nitrosodi-n-butylamine N-nitrosopiperidine N-nitrosopyrrolidine N-nitrosomorpholine N-nitroso-n-methyl-n-phenylamine N-nitrosodiphenylamine

10

56

7

8 9

TKG 1006 317

Chemical Biological & Industrial SODIUM CYCLAMATE IMPURITIES

PHENOL IN RESINS

Column: TRB-5A, P/N TR-210533 Dimensions: 30m x 0.32mm x 0.5 µm Injection: 1 µL (50-500 ppm), split 1:15, 280ºC Carrier gas: He, constant pressure 17 psi (117.1 KPa) Oven program: 85ºC (1 min)@ 8ºC/min to 150ºC(10min) @ 30ºC/min to 220ºC(5min) Detector: FID, 280ºC

Column: TRB-624, P/N TR-603035 Dimensions: 30m x 0.53mm x 3.0 µm Injection: 1 µl (0,5,5 and 50ppm), split 1:5, 260ºC Carrier gas: He, constant pressure 5 psi (34.5 KPa). Oven temperature: 150ºC (isothermal) Detector: FID, 280ºC 1

Peak Name 1 2 3 4 5 6

1 2

Peak Name

Methylene chloride Cyclohexylamine Aniline Cycloheptylamine Tetradecane Dicyclohexylamine

1 2

2

Phenol Anisol

3 4 5

1

2

6 1

2

TKG 1007

TKG 1008

SEPARATION OF SOLVENTS 14

8

Column: TRB-1, P/N TR-115063 Dimensions: 60m x 0.32mm x 5.0 µm Injection: wet needle (solvent mixture), split 1:100, 280ºC Carrier gas: He, constant pressure 14 psi (96.5 KPa). Oven program: 45ºC(7min) @ 6ºC/min to 260ºC(5min) Detector: FID, 300ºC

18 6

1

2

9

Peak Name

12

13

34

19

15 17

5

7

10

16

11

19

20

1234567891011121314151617181920-

Methanol Ethanol Acetone Isopropanol Methyl acetate Methylethyl ketone Ethyl acetate n-Butanol Isobutanol 2-Methoxypropanol Ethyleneglycol Methyl isobutyl ketone Isobutyl acetate Toluene Butyl acetate Dicetone alcohol 2-Methoxypropanol acetate Xylene Butyl glycol Butyl glycol acetate

TKG 1009 318

Chemical Biological & Industrial SEPARATION OF SOLVENTS

CHLOROFORM IMPURITIES

Column: TRB-1, P/N TR-115065 Dimensions: 60m x 0.53mm x 5.0 µm Injection: 0,1 µl solvent mix, split, 250ºC Carrier gas: H2, constant pressure 6.5 psi (45 KPa). Oven program: 40ºC (10min) @ 5ºC/min to 200ºC(15min) Detector: FID, 280ºC 20

Column: TRB-624, P/N TR-603035 Dimensions: 30m x 0.53mm x 3.0 µm Injection: 1 µl split 1:6, 260ºC, Liner: single tape with wool Carrier Gas: He, 3psi (20.7 KPa), 21.9cm/s (40ºC) Program temperature: 40ºC Detector: FID, 200ºC

13

14 15

19

12 9

Peak Name

11 16

8

1

17

1234567891011121314151617181920-

18

10

2

Peak Name

6

34

7 5

Methanol Ethanol Acetone Isopropanol Methyl acetate Methylethyl ketone Ethyl acetate Isobutanol n-Butanol 2-Methoxypropanol Ethyl glycol Methyl isobutyl ketone Isobutyl acetate Toluene Buthyl acetate Diacetone alcohol p,m-Xylenes o-Xylene AMP Butyl glycol

12345678910-

Methyl chloride ? Amylene 1,1-Dichloroethylene Methylene chloride 1,1-Dichloroethane Cis-1,2-dichloroethylene Bromochloromethane Chloroform Carbon tetrachloride

9

3 1 2

4

5

6

7

8

10

TKG 1010

TKG 1012

CHLOROFORM IMPORITIES

IMPURITIES OF n-BUTANOL

Column: Meta.VOC, P/N TR-943035 Dimensions: 30m x 0.53mm x 3.0 µm Injection: 1 µl chloroform , split, 5:1, 150ºC Carrier gas: He, constant pressure 2 psi (13.8 KPa), 19.53 cm/s (30ºC) Oven program: 30ºC (isothermal) Detector: FID, 200ºC

Column: TRB-5, P/N TR-120232 Dimensions: 30m x 0.25mm x 0.25 µm Injection: 1 µL n-Butanol, split 1:20, 250ºC Carrier gas: He, constant flow 1 mL/min Oven temperature: 40ºC @ (5min) @ 4ºC/min to 200ºC @ 15ºC/min to 300ºC Detector: MS, 280ºC (interphase) Chromatogram provided by F. Sisteré from IUCT

4

1

2

Peak Name

Peak Name

123456-

1234567-

stabilizer Methylene chloride 1,1 Dicholoroethane chloroform bromochloromethane carbon tetrachloride

Butyl acetate Xylene n-Butylether 4-Heptanol Isobutylether 2-Ethylhexanal Butyl butyrate

3 5

6

TKG 1013

TKG 1035 319

Chemical Biological & Industrial ALCOHOL IMPURITIES Column: TRB-624, P/N TR-601833 Dimensions: 30 m x 0.32 mm x 1.8 µm Injection: 0.02 µL Standard TK (3ng each compound), split 75:1, 260ºC Carrier gas: He, 23.07cm/s (40ºC) Oven temperature: 40ºC (12min) @ 10ºC/min to 200ºC (10min) Detector: FID, 260ºC Peak Name 1. Acetaldehide 2. Methanol 3. Ethanol 4. Acetone 5. 2-Propanol (Isopropanol) 6. Allyl alcohol 7. n-Propanol 8. Ethyl acetate 9. 2-Butanol

10. Cyclohexane 11. Benzene 12. Isobutanol 13. n-Butanol 14. Acetal 15. 3-Methyl-1-butanol (Isoamyl alcohol) 16. 2-Methyl-1-butanol (Amyl alcohol) 17. 4-Methyl-2-pentanol 18. Ethyl butyrate

TKG 1037

ALCOHOL IMPURITIES Column: TRB-624, P/N TR-601462 Dimensions: 60 m x 0.25 mm x 1.4 µm Injection: 0.02 µL Standard TK (4ng each compound), split 50:1, 260ºC Carrier gas: He, 26.9cm/s (40ºC) Oven temperature: 40ºC (12min) @ 10ºC/min to 240ºC (10min) Detector: FID, 260ºC Peak Name 1. Acetaldehide 2. Methanol 3. Ethanol 4. Acetone 5. 2-Propanol (Isopropanol) 6. Allyl alcohol 7. n-Propanol 8. Ethyl acetate 9. 2-Butanol 10. Cyclohexane 11. Benzene 12. Isobutanol 13. n-Butanol 14. Acetal 15. 3-Methyl-1-butanol (Isoamyl alcohol) 16. 2-Methyl-1-butanol (Amyl alcohol) 17. 4-Methyl-2-pentanol 18. Ethyl butyrate

TKG 1263 320

Chemical Biological & Industrial SEPARATION OF SOLVENTS Column: TRB-624, P/N TR-603075 Dimensions: 75m x 0.53mm x 3.0 µm Injection: 0.2 µL, split 1:5, 260ºC Carrier gas: H2, constant pressure 7.8 psi (53.74 KPa). Oven temperature: 40ºC(5min) @ 7ºC/min to 240ºC Detector: FID, 280ºC

14

13 6

15

11 7 3 9

Peak Name

16

12

5

2

12345678910111213141516-

10

8

1

1

Heptane Acetone Tetrahydrofurane Ethyl acetate Isopropyl acetate Methanol MEK Isopropanol Ethanol n-Propyl acetate MIBK n-Propanol Toluene Butyl acetate Methoxypropanol Methoxypropyl acetate

TKG 1042

SEPARATION OF SOLVENTS

IMPURITIES OF ETHANOL

Column: TRB-WAX, P/N TR-141253 Dimensions: 50m x 0.32mm x 1.2 µm Injection: 1 µL standard (500 ng/mL comp.), split 1:25, 260ºC Carrier gas: He, constant pressure 12 psi (82.7 Kpa) Oven temperature: 65ºC(7min) @ 4ºC/min to 117ºC Detector: FID, 260ºC

Column: TRB-G43, P/N TR-163035 Dimensions: 30m x 0.53mm x 3.0 µm Injection: 1 µL standard alcohols (20 ppm/comp), split 1:5, 200ºC Carrier gas: He, constant pressure 2.6 psi (17.9 KPa). Oven temperature: 42ºC(4min) @ 5ºC/min to 140ºC(4min) Detector: FID, 200ºC

Peak Name

Chromatogram provided by Jaume Piedrabuena from Danisco

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

13 2

3 7 4

1

5 6

8

9

10

11 12

Heptane (isomers mixture) Acetone Tetrahydrofurane Ethyl acetate Isopropyl acetate Methanol MEK Isopropanol Ethanol n-Propyl acetate MIBK n-Propanol Toluene Butyl acetate Methoxypropanol Methoxypropyl acetate

Peak Name 2

12345678910-

Methanol Ethanol Isopropanol n-Propanol Ethyl acetate 2-Butanol Isobutanol 1-Butanol Amylics alcohols Ethyl butyrate

14 15

3 16

TKG 1052

1

4

56

7

8

9

10

TKG 1054 321

Chemical Biological & Industrial SEPARATION IMPURITIES OF BUTYL ACRYLATE

SEPARATION OF HYDROCARBONS (FAST CHROMATOGRAPHY)

Column: TRB-1, P/N TR-111052 Dimensions: 50m x 0.25mm x 1.0 µm Injection: 0.2 µL solvent mixture, split 1:50, 280ºC Carrier gas: He, constant pressure 20 psi (137.8 KPa). Oven program: 140ºC (Isothermal) Detector: FID, 300ºC

Column: TRB-1, P/N TR-110441 Dimensions: 10m x 0.10mm x 0.40 µm Injection: 0.5 µL standard Hydrocarbons (0.95%/comp. in 2,2,4-Trimethylpentane), split 1:200, 200ºC Carrier gas: He, constant pressure 40 psi (275.6KPa). Oven temperature: 190ºC (Isothermal) Detector: FID, 200ºC Chromatogram provided by J.I. Gómez Cívicos, MªA. Uguina Zamorano and J.L. Sotelo Sancho from Universidad Complutense de Madrid

2

3 4

1

Peak Name

Peak Name

1234-

1234567-

n-Butanol Butyl formate Butyl acetate Butyl acrylate 1

2 3

n-Pentane 2,2,4-Trimethylpentane Ethylbenzene 1,3,5-Trimethylbenzene Decane Tetradecane Hexadecane

5

4

6 7

TKG 1057

TKG 1056

CHLOROFORM PURITY

POLYWAX 655

Column: TRB-5, P/N TR-121063 Dimensions: 60m x 0.32mm x 1.0 µm Injection: 250ºC, 2 µL (split 20:1) Carrier gas: H2, 11 psi (75,8 kPa). Oven temperature: 40ºC (8 min)to 200ºC(5min) @ 10ºC/min Detector: FID, 250ºC

Column: TRB-5ht, P/N TR-620112 Dimensions: 15m x 0.32mm x 0.1 µm Injection: 0, 2 µL (split) 2% Polywax 655 in Carbon sulfide Oven program: 70ºC to 250ºC @ 70ºC/min. to 395ºC(10min) @ 5ºC/min. Detector: FID, 410ºC (base line without compensation)

4

Peak Name 12345-

1 2

Chloromethane Amylene Methylene chloride Chloroform Carbon tetrachloride

5

3

TKG 1062 322

TKG 1065

Chemical Biological & Industrial IMPURITIES OF p-PHENETIDINE

DRUGS IN URINE

Column: TRB-5A, P/N TR-210532 Dimensions: 30m x 0.32mm x 0.50 µm Injection: p-Phenetidine wet needle, split 1:50, 260ºC Carrier gas: H2, 11 psi (69 KPa) Oven temperature: 80ºC(5min) @ 7ºC/min to 260ºC (6min) Detector: FID, 300ºC

Column: TRB-5ms, P/N TR-520129 Dimensions:25m x 0.20mm x 0.11 µm Injection: 250ºC, 1 µl splitless (BSTFA Derivatives in ACN) Carrier gas: He, 15 psi (103.3 kPa) Oven temperature: 60ºC (1´) to 180ºC (1´) @ 10ºC/min. to 220ºC @ 10ªC/min. Detector: FID,280ºC Chromatogram provided by Jordi To, Hospital Clínico from Barcelona. Peak Name

2 1

12345-

p-Phenetidine

Ecgonina methylester Ecgonina ethylester Cocaine Ethylcocaine Benzoilecgonina 4

Impurities 3 Impurities

5

TKG 1067

TKG 1090

AMANTADINE HYDROCHLORIDE IMPURITIES

ETHANOLAMINES SEPARATION (25 ng/peak level)

Column: TRB-5 AMINE, P/N TR-211035 Dimensions: 30m x 0.53mm x 1.0m Injection: 2 µl (split 1:50), 220ºC Carrier gas: He, 4.2 psi (28.9 kPa) Oven temperature: 70ºC (5´) to 250ºC (20min) @ 10ºC/min. Detector: FID, 300ºC Sample: Test soslution according to USP 25

Column: TRB-5 AMINE, P/N TR-210533 Dimensions: 30m x 0.32mm x 0.50 µm Injection: 2 µl (split 1:50), 280ºC Carrier gas: H2, 7 psi (48.2 kPa) Oven temperature: 50ºC (2´) to 200ºC @ 10ºC/min. Detector: FID, 300ºC Sample: Ethanolamines solution in methanol (1,25 mg/ml)

3

1

R (2-3) < 20 Impurities < 1% Peak Name 1Dichloromethane 2Adamantane 3Amantadine 4,5,6,7,8- Impurities

2

4 8 5 67

TKG 1074

TKG 1075 323

Chemical Biological & Industrial USP SOLVENTS COLUMN TRB-G27+GUARDCOLUMN 5M Column: TRB-G27, P/N-175035 Dimensions: 30m x 0.53mm x 5.0 µm Oven temp.: 35ºC(5´) to 175º[email protected] 8ºC/min. to 260ºC (16´)@35ºC/min. Carrier gas: He, 4.5 psi (31 KPa), 35 cms. to 35ºC Injector temp: 70ºC FID temp: 260ºC Injection: Direct injection of 1 µl (Unililner), standard disolution in distilled water (1:10)

12345-

ANALYSIS OF CYCLOSILOXANES 6

1

5

7

4

Column: TRB-5, P/N TR-120232 Dimensions: 30m x 0.25mm x 0.25 µm Injection: 1 µL standard (5mg/mL), 260ºC Carrier gas: H2, 12 psi (82.7 KPa) Oven temperature: 200ºC @ 10ºC/min to 325ºC(30min) Detector: FID, 340ºC

Standard

Concentration

Peak Name

Methylene chloride Chloroform Benzene Trichloroethylene 1,4 - Dioxan

600ppm 60ppm 2ppm 80ppm 380ppm

1234-7-

Methylene chloride Cis-Trimethyltriphenylcyclotrisiloxane Trans-Trimethyltriphenylcyclotrisiloxane Tetramethyltetraphenylcyclotetrasiloxane (mixture of isomers) 8-11- Pentamethylpentaphenylcylopentasiloxane (mixture of isomers) 9 10 11

5 3

1

2

3

8

2

4

TKG 1076

TKG 1094

EPA 601/602 PURGEABLE HALOCARBONS MIX PLUS 2-CHLOROETHYL VINYL ETHER Column: TRB-624, P/N TR-601462 Dimensions: 60m x 0.25mm x 1.4 µm Injection: 0.5 µL EPA 601/602 Purgeable Halocarbons Mix (2000 ng/mL), split 1:50, 260ºC Carrier gas: He 30cm/s, constant pressure 35 psi (241.15 KPa) Oven temperature: 40ºC(3min) @ 8ºC/min to 90ºC(4min) @ 6ºC/min to 200ºC(5min) Detector: FID, 260ºC

Peak Name 123456789101112131415161718192021222324252627-

Methanol 1,1-Dichloroethylene Methylene chloride trans-1,2-Dichloroethylene 1,1-Dichloroethane Chloroform 1,1,1-Trichloroethane Carbon Tetrachloride Benzene 1,2-Dichloroethane Trichloroethylene 1,2-Dichloropropane Bromodichloromethane 2-Chloroethyl vinyl ether cis-1,3-Dichloropropene Toluene trans-1,3-Dichloropropene 1,1,2-Trichloroethane Tetrachloroethylene Dibromochloromethane Chlorobenzene Ethylbenzene Bromoform 1,1,2,2-Tetrachloroethane 1,3-Dichlorobenzene 1,4-Dichlorobenzene 1,2-Dichlorobenzene

TKG 1093 324

Chemical Biological & Industrial SOLVENTS IN WATER

INDUSTRIAL SOLVENTS

Column: TRB-5, P/N TR-120232 Dimensions: 30m x 0.25mm x 0.25 m Injection: 1 µL, split Carrier gas: He Oven temperature: Detector: FID

Column: TR-WAX, P/N TR-141253 Dimensions: 50m x 0.32mm x 1.2 µm Injection: 0.1 µL, split Carrier gas: H2, 16 psi (110.24 KPa) Oven temperature: 60ºC @ 2ºC/min to 125ºC Detector: FID, 250ºC

Chromatogram provided by J. Teixidor and E. Bosch from Laboratory Dr. Riera Peak Name 1234567-

3

Peak Name

Butyl acetate Ethyl benzene m,p-Xylene o-Xylene Trimethyl benzenes n-Decane Tetramethyl benzenes

1

123456789101112-

4 8 2

3 5

9 6

10

11

12

6

Acetone Ethyl acetate MEK Isopropyl acetate Isopropanol Ethanol MIBK Methoxypropyl acetate n-Propanol Toluene Methoxypropanol Propyl acetate

7 2

4

5

7

1

TKG 1160

TKG 1161

INDUSTRIAL SOLVENTS

INDUSTRIAL SOLVENTS IN RAW MATERIALS

Column: TR-WAX, P/N TR-141233 Dimensions: 30m x 0.32mm x 1.2 µm Injection: 0.1 µL, split Carrier gas: He, 12 psi (82.7 KPa) Oven temperature: 40ºC @ 1ºC/min to 70ºC @ 7.5ºC/min to 125ºC Detector: FID, 250ºC

Column: TRB-5, P/N TR-125035 Dimensions: 30m x 0.53mm x 5.0 µm Injection: 1 µL, head space Carrier gas: N2, 5 mL/min Oven temperature: 40ºC(5min) @ 3ºC/min to 110ºC Detector: FID

1

Peak Name

Peak Name

123456-

1234567-

Acetone Methanol Ethanol p-Xylene m-Xylene o-Xylene

Acetonitrile Methylene chloride Chloroform Benzene Trichloroethylene Dioxane Pyridine

4

2 3

4 5

6

2 5

3 1

TKG 1162

6

7

TKG 1163 325

Chemical Biological & Industrial IMPURYTIES IN RAW MATERIALS Analysis of Monochloroacetic acid

AROMATIC SOLVENTS Column: TRB-WAX, P/N TR-141233 Dimensions: 30m x 0.32mm x 1.2 µm Injection: split Carrier gas: He, 10 psi (68.9 KPa) Oven temperature: 80ºC (Isothermal) Detector: FID, 250ºC

Column: TRB-5, P/N TR-120233 Dimensions: 30m x 0.32mm x 0.25 µm Injection: splitless 1 min, 260ºC Carrier gas: He, 8 psi Oven temperature: 30ºC(12min) @ 10ºC/min to 250ºC Detector: FID, 260ºC

Chromatogram provided by E. Cura from SGS, S.A., Barcelona.

Chromatogram provided by A. Tintó from MOEHS, S.A., Barcelona. Peak Name 12-

Peak Name 1

Methyl chloroacetate Internal Standard

123456-

2

Benzene Toluene Ethyl benzene p-Xylene m-Xylene o-Xylene

4

2 3

5

6

1

TKG 1164

TKG 1165

MIXTURE OF SOLVENTS

GLYCOLS IN WATER

Column: TRB-WAX, P/N TR-141233 Dimensions: 30m x 0.32mm x 1.2 µm Injection: split Carrier gas: He, 10 psi (68.9 KPa) Oven temperature: 75ºC (Isothermal) Detector: FID, 250ºC

Column: TRB-FFAP, P/N TR-150535 Dimensions: 30m x 0.53mm x 0.5 µm Injection: 1 µL, split Carrier gas: H2, 2 psi (13.8 KPa) Oven temperature: 100ºC @ 10ºC/min to 220ºC Detector: FID

Chromatogram provided by E. Cura from SGS, S.A., Barcelona. 1

Peak Name

5

1234567-

3 2

4

Acetone Methanol Isopropanol MIBK Toluene Butyl acetate Isobutanol

1

2

Peak Name 3

123-

Butanediol Propylene glycol Glycerine

6 7

TKG 1166 326

TKG 1167

Chemical Biological & Industrial PURITY OF CHLOROFORM

IMPURITIES OF DIMETHYLACETAMIDE

Column: TRB-5, P/N TR-121063 Dimensions: 60m x 0.32mm x 1.0 µm Injection: 2 µL, split, 260ºC Carrier gas: H2, 11 psi (75.8 KPa) Oven temperature: 40ºC(8min) @ 10ºC/min to 200ºC(5min) Detector: FID, 260ºC

Column: TRB-WAX, P/N TR-140232 Dimensions: 30m x 0.25mm x 0.25 µm Injection: 0.3 µL, split, 260ºC Carrier gas: H2, 11 psi (78.8 KPa) Oven temperature: 75ºC(7min) @ 10ºC/min to 200ºC Detector: FID, 280ºC Peak Name 123456-

Peak Name 12345-

Methyl chloroform Amylene Methylene chloride Chloroform Carbon tetrachloride

Methanol Dimethylformamide Dimethylacetamide Propylene glycol Ethylene glycol Monomethyl acetamide

TKG 1168

TKG 1169

IMPURITIES OF LACTIC ACID

ALCOHOLS IN BLOOD

Column: TRB-FFAP, P/N TR-151035 Dimensions: 30m x 0.53mm x 1.0 µm Injection: 0.5 µL, split, 260ºC Carrier gas: H2, 3 psi (20.7 KPa) Oven temperature: 45ºC(15min) @ 8ºC/min to 240ºC(15min) Detector: FID, 280ºC

Column: TRB-G43, P/N TR-163035 Dimensions: 30m x 0.53mm x 3.0 µm Injection: 1 µL, split, alcohols standard Carrier gas: H2, 4 psi (27.6 KPa) Oven temperature: 35ºC (isothermal) Detector: FID, 250ºC

Peak Name 12345-

TKG 1170

Methanol Ethanol Acetone Isopropanol n-Propanol (I.St.)

TKG 1172 327

Chemical Biological & Industrial IMPURITIES IN SOLVENTS Column: TRB-1, P/N TR-110352 Dimensions: 50m x 0.25mm x 0.33 µm Injection: 1 µL, split, neat solvent Carrier gas: H2, 19 psi (130.9 KPa) Oven temperature: 35ºC(5min) @ 6ºC/min to 150ºC(5min) Detector: FID, 275ºC

(Carbon tetrachloride)

(Perchloro ethylene)

TKG 1171

POLLUTANTS IN BLOOD Column: MetaBLOOD 1, P/N TR-853035 Dimensions: 30m x 0.53mm x 3.0µm Injection: 1 mL Head Space 2t (vial 70ºC), alcohols and aromatics in blood (2-20 ppm), split 1:30, 225ºC Carrier gas: He, 5 psi Oven temperature: 45ºC(7 min) @ 10ºC/min to 90ºC(10min) Detector: FID, 300ºC Chromatogram provided by Dra. Guadalupe Montoya and Dra. Isabel Bonaparte de General Lab (Barcelona)

Peak Name 1 2 3 4 5 6 7 8 9 10 11 12

Methanol Ethanol Isopropanol Acetone n-Propanol Methylethylketone (MEK) Benzene Toluene Methylisobutylketone (MIBK) Ethylbenze Xylene Styrene

TKG 1210 328

Chemical Biological & Industrial POLLUTANTS IN BLOOD Column: MetaBLOOD 1, P/N TR-853035 Dimensions: 30m x 0.53mm x 3.0µm Injection: 1 mL Head Space 2t (vial 70ºC), alcohols and aromatics in blood (2-20 ppm), split 1:30, 225ºC Carrier gas: He, 5 psi Oven temperature: 45ºC(7 min) @ 10ºC/min to 90ºC(10min) Detector: FID, 300ºC Chromatogram provided by Dra. Guadalupe Montoya and Dra. Isabel Bonaparte de General Lab (Barcelona)

Peak Name 1 2 3 4 5 6 7 8

Methanol Acetaldehyde Ethanol Isopropanol Acetone n-Propanol Methylethylketone (MEK) Methylisobutylketone (MIBK)

TKG 1213

ALCOHOLS IN BLOOD Column: MetaBLOOD 2, P/N TR-862035 Dimensions: 30m x 0.53mm x 2.0 µm Injection: 1 mL Head Space 2t, alcohols standard, split 1:10, 250ºC Carrier gas: He, 80 cm/s (40ºC) Oven temperature: 40ºC (Isothermal) Detector: FID, 260ºC

1

4

2

3

Peak Name 1 2 3 4 5 6

5

Acetaldehyde Methanol Ethanol Acetone Isopropanol n-Propanol

6

TKG 1192 329

Chemical Biological & Industrial IMPURITIES IN TOLUENE Column: TRB-PAG, P/N TR-550232 Dimensions: 30m x 0.25mm x 0.25µm Injection: 1 µL Toluene Standard, split 1:50, 260ºC Carrier gas: He, constant pressure 11 psi (75.8 Kpa) Oven Temperature: 40ºC @ 6ºC/min to 230ºC(5min) Detector: FID, 260ºC Peak Name

2

Response

1 2 3 4 5 6 7 8 9 10 11

Benzene Toluene Ethylbenzene m-Xylene p-Xylene o-Xylene Cyclohexanone 2-Methylcyclohexanone 3-Methylcyclohexanone 4-Methylcyclohexanone Cyclohexanol

1 3

4

6

5

7

8

9 10

11

Time

TKG 1194

IMPURITIES IN CYCLOHEXANE Column: TRB-1, P/N TR-111226 Dimensions: 25m x 0.15mm x 1.2 µm Injection: 1µL Cyclohexane, split 1:100, 260ºC Carrier gas: H2, constant pressure 22 psi (0.8 mL/min) Oven temperature: 60ºC(6 min) @ 20ºC/min to 160ºC(2 min) Detector: FID, 300ºC

Peak Name 1 2 3 4 5 6 7

Response

5

n-Pentane n-Hexane Methylcyclopentane Benzene Cyclohexane n-Heptane Methylcyclohexane

6

1

2

3

7 4

Time TKG 1195 330

Chemical Biological & Industrial MIXTURE OF SOLVENTS AND ISOMERS OF N-HEPTANE Column: TRB-624, P/N TR-603075 Dimensions: 75m x 0.53mm x 3.0 µm Injection: mixture of solvents (wet needle), split 1:100, 250ºC Carrier gas: He, constant pressure 8 psi (55.7 Kpa) Oven temperature: 40ºC(15 min) @ 15ºC/min to 75ºC(15 min) Detector: FID, 250ºC 5

1

Peak Name 1 2 3 4 5 6

2

Methanol Acetone Isopropanol Ethyl acetate Tetrahydrofurane Isomers n-heptane

4

3

6

TKG 1201

PETROL

Peak Name

Column: TRB-PETROL, P/N TR-110592 Dimensions: 100m x 0.25mm x 0.50µm Injection: 0.1µL petrol, split 100:1, 280ºC Carrier gas: H2, constant pressure 221Kpa (35ºC) Oven temperature:35ºC(18min) @ 2ºC/min to 200ºC(5min) Detector: FID, 280ºC

1 2 3 4 5 6 7 8 9 10 11 12 13

3

4

6

14 15 16 17 18 19 20 21 22 23 24 25

isobutane n-butane isopentane pentane 2,3-dimethylbutane 2-methylpentane 3-methylpentane hexane 2,4-dimethylpentane benzene 2-methylhexane 3-methylhexane n-heptane

toluene ethylbenzene m-xylene p-xylene o-xylene 1-methyl-3-ethylbenzene 1,3,5-trimethylbenzene 1,2,4-trimethylbenzene 1,2,3-trimethylbenzene naphtalene 2-methylnaphtalene 1-methylnaphtalene

14 21

2

7 16 19

10

8

20

5 11 9 1

18

12 13

15

17 22 23

2425

TKG 1203 331

Chemical Biological & Industrial ALCOHOLS IN BLOOD Column: MetaBLOOD 2, P/N TR-862035 Size: 30m x 0.53mm x 2.0µm Carrier gas: He, 5 psi OvenTemperature: 45ºC (15 min) Injection: 1 mL Head Space 2t (vial 70ºC), alcohols in blood (2-20 ppm), split 1:20, 225ºC Detector: FID, 300ºC

Peak Name 1 2 3 4 5 6 7 8

Chromatogram provided by Dra. Guadalupe Montoya y Dra. Isabel Bonaparte from General Lab (Barcelona)

Acetaldehyde Methanol Ethanol Acetone Isopropanol n-Propanol Methylethyl ketone (MEK) Methylisobutylketone (MIBK)

TKG 1209

AMINES Column: TRB-624, P/N TR-603065 Size: 60m x 0.53mm x 3.0µm Injection: 1 µl amines standard, split 1:5, 260ºC Carrier Gas: He, 8 mL/min Program temperature: 40ºC (1min) @ 5ºC/min to 260ºC (10min) Detector: FID KONIK-TECH, 270ºC

Sample PM (1-methoxy-2-propanol) DMEA (N,N-dimethylethanolamine) BDMA (N,N-dimethylbenzylamine) PMDTA (pentamethyldiethylentriamine) TDMAPA (N,N,N-tris(3-dimethylaminopropyl)amine)

Chromatogram provided by Ariadna Galve from KONIK-TECH, S.A

TKG 1214 332

Chemical Biological & Industrial AMINOACIDS Column: TRB-50ht, P/N TR-531332 Size: 30m x 0.25mm x 0.15µm Injection: 2 µl standard AA-S-18 Sigma (2.5µmol/ml), split, 300ºC Carrier gas: He, 1mL/min Program temperature: 50ºC (2min) @ 30ºC/min to 350ºC (3min) Detector: MS Polaris Q, EI, 200ºC, transfer line 200ºC

Peak Name 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

Chromatogram provided by Antonio Tintó from Moehs S.A.

Alanine Glycine Valine Leucine Isoleucine Serine Threonine Proline Hydroxyproline Methionine Phenylalanine Lysine Histidine Tyrosine Cystine

TKG 1215

HYDROCARBONS Column: TRB-1ht, P/N TR-610133 Size: 30m x 0.32mm x 0.1µm Injection: hydrocarbons standard1250 ppb, splitless, 250ºC Carrier gas: He, constant flow 2 mL/min Program Temperature: 50ºC(1 min) @ 40ºC/min to 320ºC(5 min) Detector: MS, ion source 200ºC, Interfase 280ºC, scan 20-600

Peak Name 1 2 3 4 5 6

Chromatogram provided by Vanesa Riu de ILERSAP, Mollerussa (Lleida).

C10 C12 C24 C28 C30 C40

2 4

6 5

1 3

TKG 1221 333

Chemical Biological & Industrial VOLATIL SOLVENTS NO HALOGENATED

Peaks

Column: TRB-1, P/N TR-111062 Size: 60m x 0.25mm x 1.0µm Injection: 1 mL Headspace (70ºC, 20min), split 1:5, 250ºC Carrier Gas: He, constant flow 1mL/min Program Temperature: 40ºC(2 min) @ 8ºC/min to 240ºC(10 min) Detector: MS, ion source 200ºC, Interfase 250ºC, scan 20-400

1 2 3 4 5 6 7 8 9 10 11 12 13

Chromatogram provided by Vanesa Riu de ILERSAP, Mollerussa (Lleida).

Isobutyl acetate Benzene Cyclohexane 3-Pentanone Propyl acetate Methyl isobutyl ketone Isobutyl acetate Toluene Butyl acetate Ethylbenzene m,p-Xylene o-Xylene Isobutyl Ketone

11

10

13 12

8

2 7

3

9 1 4

5

6

TKG 1220

BACTERIAL ACID METHYL ESTERS MIX Column: TRB-1MS, P/N TR-510232 Size: 30m x 0.25mm x 0.25µm Injection: 1µL Bacterial Acid Methyl Esters Mix (10µg/µL), split 1:100, 275ºC Carrier Gas: H2, 12 psi (41 cm/s) Program temperature: 150ºC(4min) @ 4ºC/min to 250ºC(5min) Detector: FID, 275ºC

Peaks 1234567891011121314151617181920212223242526-

Methyl undecanoate Methyl 2-hydroxydecanoate Methyl dodecanoate Methyl tridecanoate Methyl 2-hydroxydodecanoate Methyl 3-hydroxydodecanoate Methyl tetradecanoate Methyl 13-methyltetradecanoate Methyl 12-methyltetradecanoate Methyl pentadecanoate Methyl 2-hydroxytetradecanoate Methyl 3-hydroxytetradecanoate Methyl 14-methylpentadecanoate Methyl cis-9-hexadecenoate Methyl hexadecanoate Methyl 15-methylhexadecanoate Methyl cis-9,10-methylenehexadecanoate Methyl heptadecanoate Methyl 2-hydroxyheptadecanoate Methyl cis-9,12-octadecadienoate Methyl cis-9-octadecenoate Methyl trans-9-octadecenoate Methyl octadecenoate Methyl cis-9,10-methyleneoctadecenoate Methyl nonadecenoate Methyl eicosanoate

TKG 1231 334

Chemical Biological & Industrial RESIDUAL SOLVENTS IN DMSO Column: TRB-624, P/N TR-601863 Size: 60m x 0.32mm x 1.8µm Injection: 1µL mixture of solvents (500 ppm in DMSO), split 1:50, 260ºC Carrier gas: He, constant pressure 16 psi Program Temperature: 40ºC(5 min) @ 2ºC/min to 60ºC @ 9ºC/min to 115ºC @ 35ºC/min to 220ºC(15min) Detector: FID, 260ºC

Peak Name 1234567891011121314151617181920212223242526-

Methanol Ethanol Acetone 2-Propanol Acetonitrile Methylene chloride 2-Methyl-2-Propanol MTBE Hexane 1-Propanol 2-Butanone Ethyl acetate 2-Butanol Tetrahydrofuran Cyclohexane Isopropylacetate Heptane 2-Ethoxyethanol MIBK Toluene 1-Pentanol DMF Ethyl benzene m,p-Xylene o-Xylene Dimethyl sulfoxide

TKG 1232

COMMON INDUSTRIAL SOLVENTS Column: TRB-1, P/N TR-111033 Size: 30m x 0.32mm x 1.0µm Injection: 0.01µL Neat solvents, split 1:300, 200ºC Carrier Gas: H2, 7 psi Program temperature: 30ºC @ 8ºC/min to 140ºC(2min) Detector: FID, 200ºC

Peaks 12345678910111213141516171819-

Methanol Methyl formate Ethanol Acetone Isopropanol Dichloromethane n-Propanol Methyl ethyl ketone Sec-Butanol Ethyl acetate Isobutanol Isopropyl acetate Nitropropane 1,4-Dioxane Toluene Mesityl oxide Diacetone alcohol m-Xylene Cyclohexanone

TKG 1234 335

Chemical Biological & Industrial REACTION PRODUCTS OF 1,1-DIETHOXY BUTANE

SEPARATION OF ACETIC ACID AND ACETIC ANHYDRIDE

Column: TRB-624, P/N TR-603035 Size: 30m x 0.53mm x 3.0µm Injection: 0.5 µl, split 1:5, 260ºC Carrier Gas: He, 6 psi Program temperature: 40ºC (6min) @ 30ºC/min to 200ºC (5min) Detector: FID, 260ºC

Column: TRB-1, P/N TR-115035 Dimensions: 30m x 0.53mm x 5.0 µm Injection: wet needle (solvent mixture), split 1:100, 200ºC Carrier gas: H2, constant pressure 3 psi (20.7 KPa). Oven program: 90ºC Detector: FID, 260ºC 2

Chromatogram provided by Ion Aguirre from Escuela Superior de Ingeniería de Bilbao (Spain)

1

Peak Name

Peak Name

1234-

12-

Butanal Ethanol 1,1-Diethoxy butane THF

Acetic acid Acetic anhydride

4

3

1

2

TKG 1238

TKG 1038

PETROL Column: TRB-PETROL (PONA Column), P/N TR-110592 Dimensions: 100m x 0.25mm x 0.50µm Injection: 0.5µL Naphtha Repsol, split 1:250, 250ºC Carrier gas: H2, constant flow, 30 psi (206.7 KPa) Oven temperature: 0ºC to 250ºC Detector: FID, 310ºC 11 Peak Name 7

123456789101112131415-

8

12 10

13 2 1

14 3 45

6

Propane Isobutante Butane Isopentane n-Pentane n-Hexane Benzene Cyclohexane n-Heptane Toluene n-Octane n-Nonane n-Decane n-Undecane n-Dodecane

15

9

TKG 1267 336

Food & Flavors ETHYL CARBAMATE IN WINE

TUNA OIL WITH ADDED DHA

Column: SupraWax-280, 20m x 0.18mm x 0.18µm (P/N: TR-830984) Carrier gas: Helium, 1mL/min Injection: 1µL, split (30ppb of Ethyl Carbamate), 180ºC Oven temp.: 40ºC (0.75min) to 60ºC @ 10ºC/min to 150ºC @ 3ºC/min to 220ºC(4.25min) @ 30ºC/min Detector: MS (SIM, m/z 62, 74 and 89), transfer line 220ºC

Column: SupraWax-280, 15m x 0.10mm x 0.10µm (P/N: TR-830111) Injection: 1µL Methylated sample, 280ºC, split 100:1 Carrier Gas: H2, 45 psi (310.05 KPa) Oven: 100ºC (0.5min) @ 50ºC/min to 280ºC (2min) Detector: FID, 280ºC

Chromatogram provided by Joan Garcia, INCAVI (Vilafranca del Penedés, Barcelona)

Ethyl Carbamate 30 mg/L

TKG 1260

TKG 1261

ALCOHOLS

Peak Name

Column: TRB-20, P/N TR-201235 Dimensions: 30m x 0.53mm x 1.2µm Injection: wed needle, 260ºC, split 1:100 Carrier gas: He 2.2psi Oven Temperature: 45ºC(2min) @ 10ºC/min to 100ºC Detector: FID, 260ºC

123456789101112-

Methanol Ethanol 2-Propanol Acetone 1-Propanol 2-Butanol Ethyl Acetate Isobutanol Tert-Amyl Alcohol 1-Butanol Isoamyl Alcohol 1-Pentanol

TKG 1259 367

Foods & Flavors 3 1

SEPARATION OF FAMES

SEPARATION OF FAMES

Column: TR-WAXOmega, P/N TR-840232 Dimensions: 30m x 0.25mm x 0.25 µm Injection: 1 µL 25mg FAME’s/mL in Hexane, split 1:100, 260ºC Carrier gas: H2, 12 psi (82.7 KPa) Oven temperature: 200ºC (Isothermal) Detector: FID, 260ºC

Column: TR-CN100, P/N TR-882162 Dimensions: 60m x 0.25mm x 0.20 µm Injection: 0.4 µL (10 mg/mL), split 1:100, 260ºC Carrier gas: H2, 25 psi (172.2 KPa) Oven program: 160ºC(10min) @ 1ºC/min a 220ºC(5min) Detector: FID, 260ºC

Peak Name 12345678910111213141516171819-

2 Peak Name

7

123456789101112-

1314151617181920212223242526-

C14:0 C16:0 C16:1n7 C16:2n4 C16:3n4 C18:0 C18:1n9 C18:1n7 C18:2n6 C18:4n3 C20:0 C20:1n9

C20:4n6 C20:3n3 C20:4n3 C20:5n3 (EPA) C22:0 C22:1n9 C22:2n6 C21:5n3 C23:0 C22:4n3 C22:5n3 C24:0 C22:6n3 (DHA) C24:1n9

2 12

7

14

C14:0 C16:0 C16:1c C17:0 C18:0 C18:1t C18:1c C18:2t C18:2c C20:0 C20:1c CLA911ct CLA1113 CLA1012tc CLA911cc CLA1012cc CLA tt CLA ox C22:0

5 45

8

16 10

69

17 9

11 12 13

14

15 17

21 19 20

18

23

22

24

1

25 26

3 4

6

8

13 15 16 18

TKG 1027

19

TKG 1029

ESSENTIAL OIL 10

2 3

Column: TRB-WAX, P/N TR-140262 Dimensions: 60m x 0.25mm x 0.25 µm Injection: 1.5 µL Essential oil, splitless 0.5min, 250ºC Carrier gas: He, constant pressure 26 psi (180 KPa). Oven program: 45ºC(5min) @ 2ºC/min to 230ºC(15min) Detector: FID, 280ºC

22 27

24

6

28 26

Peak Name

11

4

12345678910-

7 15

20

13 12

1

5

19

14 8

18 17

21

29 9

16

ethyl butyrate 1-Propanol isobutanol isoamyl acetate 1-butanol isoamyl alcohol ethyl hexanoate n-hexyl acetate 1-butanal ethyl lactate

11121314151617181920212223242526272829-

1-hexanol 3-ethoxy-1-propanol cis-3-hexenol furfural propionic acid isobutyric acid methyl furfural butyric acid diethyl succinate a-terpineol phenyl ethyl acetate hexanoic acid geraniol phenyl ethanol enantic acid 4-ethyl guaiacol octanoic acid decanoic acid ethyl palmitate

23

TKG 1031 368

Foods & Flavors SEPARATION OF ALCOHOLS

ANALYSIS OF CHLOROPICRINE IN WINES

Column: Meta .WAX, P/N TR-811035 Dimensions: 30m x 0.53mm x 1.0 µm Injection: 1 µL standard, split 1:4, 200ºC Carrier gas: He, 3 psi (20.7 KPa) Oven temperature: 40ºC(10min) @ 6ºC/min to 125ºC(5min) Detector: FID, 200ºC

Column: TRB-5, P/N TR-120232 Dimensions: 30m x 0.25mm x 0.25 µm Injection: 1 µL standard (5mg/L), 200ºC Carrier gas: H2, 12 psi (82.7 KPa) Oven temperature: 43ºC(7min) @ 30ºC/min to 120ºC(10min) Detector: ECD, 300ºC Peak Name

Peak Name 12345678-

2

12345-

Methanol Ethanol 2-Butanol n-Propanol Isobutanol n-Butanol 2-Methyl-1-butanol (isoamylic alcohol) 3-Methyl-1-butanol (amyl alcohol)

Monochloroisothiocyanate Methyl isothiocyanate Dichloroisothiocyanate Trichloroisothiocyanate (CHLOROPICRINE) Allyl isothiocyanate

4 5

7

1 3

4

5

8

6

1

3 2

TKG 1030

TKG 1032

SEPARATION OF VOLATILES IN ALCOHOLIC BEVERAGES Column: Meta .WAX, P/N TR-810532 Dimensions: 30m x 0.25mm x 0.5 µm Injection: 0.6 µL mixture of solvents 1% in Ethanol, split 1:100, 250ºC Carrier gas: He, 12 psi (82.7 KPa) Oven temperature: 35ºC(10min) @ 8ºC/min to 220ºC(15min) Detector: FID, 260ºC

Peak Name 12345678910111213141516171819202122-

Acetaldehyde Methyl acetate Acetal Ethyl acetate Methanol Ethanol Ethyl butyrate 2-Butanol n-Propanol Isobutanol 1-Butanol 4-Methyl-2-pentanol (I. St.) C-6-Ethyl 2-Methyl-1-butanol 3-Methyl-1-butanol Hexanol Ethyl lactate C-8-Ethyl Benzaldehyde C-9-Ethyl C-10-Ethyl 4-Ethylphenol

TKG 1026 369

Foods & Flavors SEPARATION OF ACRYLAMIDE

IDENTIFICATION OF WAX IN OLIVE OIL

Column: TRB-WAX, P/N TR-140535 Dimensions: 30m x 0.53mm x 0.5 µm Injection: 1 µL AMD (6ppm)/MAMD (33 ppm) mixture in Metanol, splitless, 250ºC Carrier gas: H2, constant pressure, 2 psi (13.78 KPa) Oven temperature: 135ºC(10min) @ 25ºC/min to 190ºC(5min) Detector: FID, 260ºC

Column: TRB-5, P/N TR-120113 Dimensions: 15m x 0.32mm x 0.1 µm Injection: 1 µL extraction of Olive Oil folowing norm, On Column, 280ºC Carrier gas: H2, 2mL/min Oven program: 85ºC @ 35ºC/min to 180ºC @ 3ºC/min to 330ºC Detector: FID, 350ºC Chromatogram provided by J.E. Trujillo from Ybarra (Sevilla)

Peak Name

Peak Name

12-

1234567-

Methacrylamide (MAMD) Acrylamide (AMD) 1

2

C32 (1.St) C36 C38 C40 C42 C44 C46

1

5

2 3

6

4

TKG 1033

7

TKG 1034

SEPARATION OF ACETOINE IN VINEGAR

SEPARATION OF POLYOLS IN WINE

Column: TRB-FFAP, P/N TR-151025 Dimensions: 25m x 0.53mm x 1.0 µm Inyection: 1 µL, split 1:100, 250ºC Carrier gas: H2 , constant pressure 2.9 psi (19.98 Kpa) Oven temperature: 100ºC(5 min) @ 10ºC/min a 200ºC Detector: FID, 250ºC

Column: TRB-FFAP, P/N TR-150262 Dimensions: 60m x 0.25mm x 0.25 µm Injection: 1 µL, split 1:100, 250ºC Carrier gas: H2 , constant flow 1mL/min Oven temperature: 80ºC(5 min) @ 3ºC/min to 200ºC(15 min) Detector: FID, 250ºC Peak Name 1234567-

Peak Name 2

12-

Acetoine Acetic acid 1

2

4

5

Methanol D(-)-2,3-Butanediol Meso-2,3-Butanediol 1,2-Propanediol Ethyleneglycol 1,3-Butanediol Glycerol 7

6

1 3

TKG 1040 370

TKG 1041

Foods & Flavors ANALYSIS OF RAPESEED OIL (FAST CHROMATOGRAPHY)

ANALYSIS OF RAPESEED OIL

Column: TRB-WAX, P/N TR-142181 Dimensions: 20m x 0.10mm x 0.2 µm Injection: 0.7 µL Rapeseed oil, split 1:500, 280ºC Carrier gas: H2, constant pressure, 54 psi (372 KPa), 41.15 cm/s Oven temperature: 205ºC (Isothermal) Detector: FID, 280ºC

Column: TRB-WAX, P/N TR-140232 Dimensions: 30m x 0.25mm x 0.25 µm Injection: 1 µL Rapeseed oil, split 1:50, 280ºC Carrier gas: H2, 36.23 cm/s Oven temperature: 205ºC (Isothermal) Detector: FID, 280ºC

4

5

Peak Name

Peak Name

1 2 3 4 5 6 7 8 9 10 11

1 2 3 4 5 6 7 8 9 10 11

Myristic acid methyl ester (C14:0) Palmitic acid methyl ester (C16:0) Stearic acid methyl ester (C18:0) Oleic acid methyl ester (C18:1) Linoleic acid methyl ester (C18:2) Linolenic acid methyl ester (C18:3) Arachidic acid methyl ester (C20:0) Cis-11-eicosenoic acid methyl ester (C20:1) Behenic acid methyl ester (C22:0) Erucic acid methyl ester (C22:1) Lignoceric acid methyl ester (C24:0)

4

Myristic acid methyl ester (C14:0) Palmitic acid methyl ester (C16:0) Stearic acid methyl ester (C18:0) Oleic acid methyl ester (C18:1) Linoleic acid methyl ester (C18:2) Linolenic acid methyl ester (C18:3) Arachidic acid methyl ester (C20:0) Cis-11-eicosenoic acid methyl ester (C20:1) Behenic acid methyl ester (C22:0) Erucic acid methyl ester (C22:1) Lignoceric acid methyl ester (C24:0)

2

5 1

3

6

2 7 8

10

6 minutes

9

1

6

3

11

7

9 10

8

11

TKG 1045

TKG 1046

ALCOHOLS AND TERPENIC ACIDS (OLIVE OIL)

ANALYSIS BERGAMOL

Column: TRB-35, P/N TR-351332 Dimensions: 30m x 0.25mm x 0.15 µm Injection: 1 µL extract of leaf of Olive Tree, split 1:20, 300ºC Carrier gas: H2, constant pressure 12 psi (82.7 KPa). Oven temperature: 275ºC Detector: FID, 300ºC

Column: Meta.WAX, P/N TR-810532 Dimensions: 30m x 0.25mm x 0.5 µm Injection: 1 µL standard 0.3% v/v Bergamol/Diethyl Phtalate in Ethanol, split 1:50, 260ºC Carrier gas: H2, 12 psi (82.7 KPa). Oven temperature: 35ºC(10min) @ 8ºC/min to 220ºC(20min) Detector: FID, 260ºC

Chromatogram provided by Angeles Guinda from Instituto de la Grasa, CSIC.

Peak Name

Peak Name

12345-

1 2-6 7

Eritrodiol Uvaol Oleanolic acid Ursolic acid Maslinic acid

Ethanol Bergamol Diethyl Phtalate

1

3

2 1

4

5

7 2 3

TKG 1049

45 6

TKG 1050 371

Foods & Flavors ALDEHYDES Column: Meta. X5, P/N TR-820232 Dimensions: 30m x 0.25mm x 0.25 µm Injection: 1 µL reaction Epoxydecenal and Butylamine, splitless (1.5min), 280ºC Carrier gas: He, constant pressure 12 psi (82.7 KPa). Oven temperature: 50ºC(1min) @ 5ºC/min to 240ºC @ 10ºC/min to 300ºC Detector: MS, 280ºC

6

2

Chromatogram provided by F. Javier Hidalgo García from Instituto de la Grasa, CSIC.

Peak Name

5

123456789-

9

3 4

Hexanal 1-Butylpyrrole Butanal 2-Octanal 3-Nonanal 2,4-Decadienal 2-Butiloctanal N-Butyl-2-pentylpyrrole N-Butyl-2-(1-hydroxyhexyl)pyrrole

8

1

7

TKG 1051

PESTICIDES IN WINES

SEPARATION OF STEROLS IN OLIVE OIL (VIRGEN EXTRA)

Column: TRB-5, P/N TR-120232 Dimensions: 30m x 0.25mm x 0.25 µm Injection: 1 µL standard in Cyclohexane, 250ºC Carrier gas: He, 1 mL/min Oven temperature: 145ºC @ 0.55ºC/min to 220ºC Detector: ECD, 300ºC

Column: TRB-1, P/N TR-111535 Dimensions: 30m x 0.53mm x 1.5 µm Injection: 1 µL extraction of Olive Oil folowing norm, splitless (1.5min), 280ºC Carrier gas: He, constant pressure 3 psi (20.7 KPa). Oven program: 265ºC (Isothermal) Detector: FID, 300ºC

Chromatogram provided by J. García from INCAVI, Vilafranca del Penedès (Barcelona)

Chromatogram provided by Jesus Rodríguez from Aceites Monterreal (Villa del Río, Córdoba)

Peak Name

1

2

8 34

12345678910111213-

Methyl parathion Vinclozoline Phenitrothion Diclofluanide Chlorpiryphos Captan Folpet Chlozolinate Procimidone Captafol Iprodione Bromopropilate Phenarimol

8 1

10 13

5 6

7 9 10

3 2

4 5 7 9 6

11 12 13

12 11

TKG 1053 372

Peak Name 1Cholesterol 224-methylenecholesterol 3Campesterol 4Stigmasterol 5δ7-campesterol 6δ5,23-stigmasterol 7Chlerosterol 8β-sitosterol 9Sitosterol 10- δ5-avenasterol 11- δ5,24-stigmastadienol 12- δ7-stigmasterol 13- δ7-avenasterol

TKG 1082

Foods & Flavors HYDROGENATED SOYA OIL Column: TR-CN100, P/N TR-882162 Dimensions: 60m x 0.25mm x 0.20 µm Injection: 0.4 µL Hydrogenated Soya Oil, split 1:50, 280ºC Carrier gas: H2, 25 psi (172.2 KPa) Oven program: 150ºC(15min) @ 1.5ºC/min to 220ºC(5min) Detector: FID, 280ºC

3

Peak Name 123-

C18:0 C18:1trans C18:1cis

1 2

TKG 1024

SEPARATION OF FAMES Column: TR-WAXOmega, P/N TR-840233 Dimensions: 30m x 0.32mm x 0.25 µm Injection: 1 µL test SP-4-8476, split 1:90, 250ºC Carrier gas: H2, 9.5 psi (65.4 KPa) Oven temperature: 200ºC (Isothermal) Detector: FID, 260ºC

1 2

Peak Name 4

9

3 12 7 5

6

15 8

12345678910111213141516-

C14:0 C16:0 C18:0 C18:1n9 C18:1n7 C18:2n6 C18:4n3 C20:0 C20:5n3 (EPA) C22:0 C21:5n3 C23:0 C22:5n3 C24:0 C22:6n3 (DHA) C24:1n9

10 11

13 14 16

TKG 1028 373

Foods & Flavors PHENOLS-ANISOL IN WINE

PHENOL-ANISOL IN WINE (FAST CHROMATOGRAPHY)

Column: TR-5MS P/N TR-520232 Dimensions: 30m x 0.25mm x 0.25 µm Injection: 280ºC, 1 µL (St 100 ppb), split (30:1) Carrier gas: H2, 1,2 ml/min. 17 psi (117 kPa) to 80ºC Oven temperature: 80ºC to 120ºC (5min) @ 10ºC/min. Detector: ECD, 330ºC

Column: META.X5 P/N TR-820441 Dimensions: 10m x 0.10mm x 0.40 µm Injection: 330ºC,0,5 µL (St 10 ppb), split (200:1) Carrier gas: H2, 40 psi (275.6 kPa) Oven temperature: 100ºC to 250ºC @ 20ºC/min. Detector: ECD, 330ºC

Peak Name 123456-

Trichlorophenol Trichloroanisole Tetrachlorophenol Tetrachloroanisole Pentachlorophenol Pentachloroanisole

Peak Name 123456-

Trichlorophenol Trichloroanisole Tetrachlorophenol Tetrachloroanisole Pentachlorophenol Pentachloroanisole

Exceptional symmetry of the peaks at traces level 12

3

4

5

5 6 12

3

4

6

TKG 1068

TKG 1193

PHYTOSANITARY ANALYSIS IN WINE Column: Meta.X5 P/N TR-820232 Dimensions: 30m x 0.25mm x 0.25 µm Injection: 2,0 µL split (1:100), 250ºC Carrier gas: He, 1 ml/min. Oven temperature: 140ºC to 180ªC @ 0,4ºC/min. to 270ºC(15min.) @1ºC/min. Detector: ECD, 300ºC, make up Argon/methane (95/5) Sample: Phytosanitary standard INCAVI, (70-680 µg/L of each component) Chromatogram supplied by M. Jaldo, J. García (Incavi) and J. Marco (Torres, S.A.)

Peak Name 12345678910111213141516171819202122-

Methylchlorpyrifos Methylparathion Vinclozoline Fenitrothion Dichlofluanide Malathion Chlorpyrifos Captan Penconazol Folpet Chlozolinate Triadimenol + Procimidione Triadimenol Hexocanozol Captafol Iprodione Bromopropylate Fenarimol Cyalotrin Fenvalerate Fenvalerate Azoxystrobine

TKG 1079 374

Foods & Flavors SEPARATION OF STEROLS IN OLIVE OIL (ORUJO)

CHLORINATED SOLVENTS IN OLIVE OIL

Column: TRB-1, P/N TR-111535 Dimensions: 30m x 0.53mm x 1.5 µm Injection: 1 µL extraction of Olive Oil folowing norm, splitless (1.5min), 280ºC Carrier gas: He, constant pressure 3 psi (20.7 KPa). Oven program: 265ºC (Isothermal) Detector: FID, 300ºC

Column: TRB-5, P/N TR-121033 Dimensions: 30m x 0.32mm x 1.0 µm Injection: 0.5 µL Head Space (2t), standard 0.05 ppm, 150ºC Carrier gas: He, 7psi (48.2 KPa) Oven temperature: 50ºC (Isothermal) Detector: ECD, 250ºC

Chromatogram provided by Jesus Rodríguez from Aceites Monterreal (Villa del Río, Córdoba) Peak Name 1Cholesterol 224-methylenecholesterol 3Campesterol 4Stigmasterol 5δ7-campesterol 6δ5,23-stigmasterol 7Chlerosterol 8β-sitosterol 9Sitosterol 10- δ5-avenasterol 11- δ5,24-stigmastadienol 1 12- δ7-stigmasterol 13- δ7-avenasterol

Peak Name 123456-

8

Chloroform 1,1,1-Trichloroethane Bromodichloromethane Dibromochloromethane Tetrachloroethylene Bromoform

3 1

4 3 2

4 5

10 6 7 9 11

2

5

6

12 13

TKG 1083

TKG 1092

ANALYSIS OF VOLATILES IN OLIVE OIL Column: TRB-WAX, P/N TR-140262 Dimensions: 60m x 0.25mm x 0.25 µm Injection: 1 µL Olive oil, SPME (SP-57329-U), 260ºC Carrier gas: H2, 1.5mL/min Oven temperature: 40ºC @ 2ºC/min to 200ºC Detector: FID, 280ºC Chromatogram provided by Departamento de Caracterización y Calidad de los Alimentos, CSIC (Sevilla)

TKG 1091 375

Foods & Flavors SEPARATION OF VOLATILES IN ALCOHOLIC BEVERAGES

ANALYSIS OF GLYCOLS IN WINE

Column: TRB-WAX, P/N TR-141035 Dimensions: 30m x 0.53mm x 1.0 µm Injection: 1 µL, split Carrier gas: He, 5 psi (34.5 KPa) Oven temperature: 40ºC @ 2ºC/min to 150ºC Detector: FID, 225ºC

Column: TRB-FFAP, P/N TR-151035 Dimensions: 30m x 0.53mm x 1.0 µm Injection: 1 µL, split Carrier gas: He, 4 psi (27.6 KPa) Oven temperature: 100ºC @ 5ºC/min to 200ºC(10 min) Detector: FID, 275ºC Peak Name 1234567891011-

Acetaldehyde Ethyl acetate Methanol Ethanol (50%) 2-Butanol 1-Propanol Isobutanol Allyl alcohol 1-Butanol Isoamyl alcohols Ethyl lactate

Peak Name 1234567-

Isoamyl alcohol Ethyl lactate Acetic acid Levo-2,3-butanediol Meso-2,3-butanediol 1,2-Propanediol Glycerine

TKG 1174

ANALYSIS OF POLYOLS IN WINE

PHENOLS EPA 604

Column: TRB-FFAP, P/N TR-150262 Dimensions: 60m x 0.25mm x 0.25 µm Injection: 1 µL, split (100:1), glycols standard, 205ºC Carrier gas: H2, 1 mL/min (80ºC) Oven temperature: 100ºC @ 5ºC/min to 200ºC(10 min) Detector: FID, 275ºC

Column: TRB-5, P/N TR-120232 Dimensions: 30m x 0.25mm x 0.25 µm Injection: 1 µL, split, 2 to 6 ng/comp, 250ºC Carrier gas: H2, 12 psi (82.68 KPa) Oven temperature: 80ºC(4min) @ 8ºC/min to 250ºC Detector: FID, 280ºC

Chromatogram provided by R. Franquet and J. García from INCAVI, Vilafranca del Penedès (Barcelona) Peak Name 123456-

Ethanol 2,3-Butanediol 1,2-Propanediol Ethylene glycol 1,3-Butanediol Glycerol

TKG 1176 376

TKG 1175

Peak Name 1234567891011-

Phenol Chorophenol 2-Nitrophenol 2,4-Dimethylphenol 2,4-Dichlorophenol 4-Chloro-3-methylphenol 2,4,6-Trichlorophenol 2,4-Dinitrophenol 4-Nitrophenol 2-Methyl-4,6-dinitrophenol Pentachlorophenol

TKG 1153

Foods & Flavors SEPARATION OF FAMES Column: TR-WAX, P/N TR-140262 Dimensions: 60m x 0.25mm x 0.25 µm Injection: 1 µL, split Carrier gas: He, 26 psi (179.1 KPa) Oven temperature: 60ºC @ 30ºC/min to 150ºC @ 2ºC/min to 240ºC Detector: FID, 275ºC

TKG 1177

SEPARATION OF METHYL ESTERS (RAPESSED OIL)

FLAVORS IN WINE

Column: TRB-WAX, P/N TR-141035 Dimensions: 30m x 0.53mm x 1.0 µm Injection: 1 µL, split Carrier gas: He, 4psi (27.6 KPa) Oven temperature: 220ºC (Isothermal) Detector: FID, 280ºC

Column: TRB-WAX, P/N TR-142168 Dimensions: 60m x 0.22mm x 0.20 µm Injection: 1 µL, split Carrier gas: He, 1 mL/min Oven temperature: 45ºC @ 5ºC/min to 230ºC Detector: FID, 250ºC Peak Name 1234567891011-

C14:0 C16:0 C18:0 C18:1 C18:2 C18:3 C20:0 C20:1 C22:0 C22:1 C24:0

TKG 1179

Chromatogram provided by M. Creixell, R. Franquet and J. Garcia from INCAVI, Vilafranca del Penedès, Barcelona.

Peak Name 12-Butanol 2Ethyl isovalerate 31-Butanol 4Ethyl caproate 5n-Hexyl acetate 6Ethyl lactate 71-Hexanol 83-Ethoxy-1-propanol 9cis-3-hexen-1-ol 10- 2-Octanol (I. St.) 11- Ethyl caprylate 12- Benzaldehyde 13- Ethyl caprate 14- γ-Butyrolactone 15- 2-Phenylethanol acetate 16- Trans-β-methyl-γ-octalactone 17- 2-Phenylethanol 18- Cis-β-methyl-γ-octalactone 19- 4-Ethylguaiacol 20- Eugenol 21- 4-Ethylphenol

TKG 1180 377

Foods & Flavors LAVANDER FLAVOR

FLAVORS (LAVANDER, ESSENTIAL OIL)

Column: TRB-WAX, P/N TR-140232 Dimensions: 30m x 0.25mm x 0.25 µm Injection: 1 µL, split Carrier gas: He, 90 KPa Oven temperature: 80ºC @ 4ºC/min to 230ºC(20 min) Detector: FID, 260ºC

Column: TRB-WAX, P/N TR-140232 Dimensions: 30m x 0.25mm x 0.25 µm Injection: 1 µL, split Carrier gas: He, 90 KPa Oven temperature: 80ºC @ 4ºC/min to 230ºC(20 min) Detector: FID, 260ºC

Chromatogram provided by C. Ibañez from Lucta, S.A, Barcelona.

Chromatogram provided by C. Ibañez from Lucta, S.A, Barcelona.

TKG 1181

TKG 1182

ANALYSIS OF PESTICIDES Column: TRB-5, P/N TR-120232 Dimensions: 30m x 0.25mm x 0.25 µm Injection: split Carrier gas: He Oven temperature: 125ºC(1 min) @ 8ºC/min to 200ºC(10 min) @ 20ºC/min to 270ºC(15 min) Detector: FPD, 280ºC Chromatogram provided by E. Casado from Laboratorio de Plagicidas of Centro Nacional de Alimentación y Nutrición, Madrid.

Peak Name 123456789101112131415161718192021-

Metacriphos Dioxathion Fonofos Diazinon Etrimfos Methyl parathion Fenclorphos Fenitrothion Malathion Ethyl parathion Ruelene Methyl bromophos Ethyl pirymiphos Isofenphos Meditathion Ethyl bromophos Gardona Ethion Trithion Fosalon Cumaphos

TKG 1183 378

Foods & Flavors STEROLS ANALYSIS (REFINATED OLIVE OIL) Column: TRB-STEROL, P/N TR-182238 Dimensions: 30m x 0.22mm x 0.22 µm Injection: split Carrier gas: H2, 20 psi (137.8 KPa) Oven temperature: 275ºC (Isothermal) Detector: FID, 300ºC Chromatogram provided by Dr. Manuel León from Instituto de la Grasa, Sevilla

I.S.

6

Peak Name 12345678910111213IS-

2

1

7 3

45

8

Cholesterol Campesterol Stigmasterol ∆5,23-Stigmastadienol Clerosterol β-Sitosterol Sitostanol ∆5-Avenasterol ∆5,24-Stigmastadienol ∆7-Stigmastenol ∆7-Avenasterol Erythoradiol Uvaol Internal St.

12

9 10 11

13

TKG 1184

ANALYSIS OF CIS-TRANS ISOMERS

ANALYSIS OF METHYL ESTERS

Column: TR-CN100, P/N TR-882133 Dimensions: 30m x 0.32mm x 0.20mm Injection: 1 µL isomers standard, split Carrier gas: H2, 4.5 psi (31 KPa) Oven temperature: 140ºC @ 4ºC/min to 190ºC Detector: FID, 250ºC

Column: TR-CN100, P/N TR-882135 Dimensions: 30m x 0.53mm x 0.20 µm Injection: 2 µL FAMES standard, split Carrier gas: He, 20 KPa Oven temperature: 130ºC(5 min) @ 3ºC/min to 160ºC Detector: FID, 250ºC Chromatogram provided by Dr. R. Garcés from Instituto de la Grasa, Sevilla.

Peak Name

Peak Name

123456-

123456-

C18:0 C18:1 trans C18:1 cis C18:2 trans C18:2 cis C20:0

C16:0 C16:1 C18:0 C18:1 C18:2 C20:0

4 1

5 3

2

6

time TKG 1185

TKG 1186 379

Foods & Flavors ANALYSIS OF METHYL ESTERS

SEPARATION OF SUGARS (AS ALDITOL ACETATES)

Column: TR-CN100, P/N TR-882113 Dimensions: 15m x 0.32mm x 0.20 µm Injection: 1 µL FAMES standard, split Carrier gas: H2, 2.4 psi (16.6 KPa) Oven temperature:140º[email protected] 3ºC/min to 185ºC Detector: FID, 250ºC

Column: TRB-225, P/N TR-250232 Dimensions: 30m x 0.25mm x 0.25 µm Injection: 1 µL Sugars standard, split Carrier gas: H2, 11 psi (75.8 KPa) Oven temperature: 220ºC (Isothermal) Detector: FID, 250ºC

Peak Name

Peak Name

12345678910-

1234-

C14:0 C16:1 C18:0 C18:1 C18:2 C20:0 C20:1 + C18:3 C22:0 C22:1 C24:0

TKG 1188

TKG 1187

SEPARATION OF SUGARS (AS ALDITOL ACETATES)

SEPARATION OF STEROLS

Column: TRB-1701, P/N TR-130212 Dimensions: 15m x 0.25mm x 0.25 µm Injection: 1 µL Sugars standard, split Carrier gas: H2, 6 psi (41.3 KPa) Oven temperature: 180ºC @ 4ºC/min to 215ºC Detector: FID, 250ºC

Column: TRB-5, P/N TR-120535 Dimensions: 30m x 0.53mm x 0.50 µm Injection: 0.1 µL Sterols standard, direct injection Carrier gas: H2, 4 psi (27.6 KPa) Oven temperature: 275ºC (Isothermal) Detector: FID, 300ºC

Peak Name

Peak Name

1234-

1234-

Rhamnitol Fucitol Ribitol Arabinitol

1

2

3

Brassicasterol Campesterol Stigmasterol β-Sitosterol

4

TKG 1189 380

Rhamnitol Fucitol Ribitol Arabinitol

TKG 1190

Foods & Flavors SEPARATION OF VOLATILES IN ALCOHOLIC BEVERAGES Column: TRB-WAX, P/N TR-141035 Dimensions: 30m x 0.53mm x 1.0 µm Injection: 0.3 µL standard, direct injection (injector of packed columns) Carrier gas: N2, 4.5 mL/min Oven temperature: 40ºC @ 2ºC/min to 110ºC Detector: FID, 250ºC

Peak Name 123456789101112-

Acetaldehyde Ethyl acetate Methanol Ethanol 2-Butanol 1-Propanol Isobutanol Allyl alcohol 1-Butanol Isoamyl alcohols Acetoine Ethyl lactate

TKG 1191-A

Distillated alcohol

Rectified alcohol

TKG 1191-B

TKG 1191-C 381

Foods & Flavors “alcohol melazas”

Schotch whisky

TKG 1191-D

Brandy 63º

Liquor

TKG 1191-F 382

TKG 1191-E

TKG 1191-G

Foods & Flavors Gin

TKG 1191-H

Jerez Vinegar

TKG 1191-I 383

Foods & Flavors EXTRACT OF ROSEMARY IN N-PENTANE Column: TRB-1, P/N TR-110141 Size: 10m x 0.10mm x 0.10µm Injection: 0.5µL, split 1:100, 300ºC (liner 1mm) Carrier Gas: He, constant pressure 35 psi (0.4mL/min) Program Temperature: 80ºC(2 min) @ 20ºC/min to 250ºC(5 min) Detector: FID, 260ºC (make up N2, 60 mL/min)

Peak Name 3 5

1 2 3 4 5

a-Pinene Camphene Eucalyptol 1,7,7-Tricamphor Verbenone

4

1 2

TKG 1196

STEROLS IN OLIVE OIL Column: TRB-STEROL, P/N TR-180738 Size: 30m x 0.22mm x 0.12µm Injection: 1 µL olive oil in diethyl ether (extraction following norm), split 1:30, 250ºC Carrier Gas: H2, constant flow 1.6 mL/min Oven Temperature: 285ºC Detector: FID, 320ºC Peak Name 1 2 3 4 5 6 7 8 9 10 11 12

Campesterol Estigmasterol D7-Campesterol D5,23-Estigmastasdienol Clerosterol b-Sitosterol D5-Avenasterol D5,24-Estigmastadienol D7-Estigmastenol D7-Avenasterol Eritrodiol Uvaol

Chromatogram provided by Dr. Manuel León, Instituto de la Grasa (Sevilla).

TKG 1197 384

Foods & Flavors TRIGLYCERIDES IN MILKY FAT Column: TRB-1ht SimDist, P/N TR-6113A5 INOX Size: 5m x 0.53mm x 0.15µm Injection: 1 µL triglycerides of milky fat standard BCR, 370ºC, split Carrier Gas: He, 15 psi Program Temperature: 200ºC(1 min) @ 6ºC/min to 350ºC(5 min) Detector: FID, 370ºC (N2 make up) Chromatogram provided by Pablo Ramos Balbona , Reny Picot (Navia, Asturias)

Peak Name 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17

CN24 Cholesterol CN26 CN28 CN30 CN32 CN34 CN36 CN38 CN40 CN42 CN44 CN46 CN48 CN50 CN52 CN54

TKG 1230

ALLERGENS IN COSMETICS Column: TRB-WAX, P/N TR-140232 Size: 30m x 0.25mm x 0.25µm Injection: 1 µl standard (25 µg/ml) in dichloromethane, splitless (60s), 250ºC Carrier Gas: He, 1mL/min Program temperature: 32ºC (5min) @ 4ºC/min to 250ºC (5min) Detector: MS KONIK-TECH, Mode EI+ (70 eV), rango mas 35-300, Scan time 35ms, Source Temperature 120ºC, Interface 250ºC, Photomultiplier 750V. Chromatogram provided by Nieves Sarrión from KONIK-TECH, S.A (Barcelona).

Peak Name 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 IS

a-Pinene Camphene β-Myrcene a-Terpinene Limonene g-Terpinene Terpinolene β-citronellal Linalool Linalyl acetate Neral s-Carvone Geranial β-Citronellol aGeraniol Benzyl alcohol Hydroxycitronellal Eugenol Thymol Cinnamyl alcohol Farnesol (1) Farnesol (2) Isoeugenol Hexyl cinnamal Farnesol (3) Coumarine Benzyl benzoate Ethyl heptanoate

TKG 1199 385

Foods & Flavors Column: TRB-WAX, P/N TR-140232 Size: 30m x 0.25mm x 0.25µm Injection: split 1:30, 250ºC (liner SPME, fibra: 2cm 50/30 µm DVB/Carboxen/PDMS) Sample: 5 µl soap in 4 mL of water (26.6% NaCl) Carrier gas: He, 1mL/min Program temperature: 32ºC (5min) @ 4ºC/min to 250ºC (5min) Detector: MS KONIK-TECH, Mode EI+ (70 eV), range mass 35-300, Scan time 35ms, Source Temperature 120ºC, Interface Temperature 250ºC, Photomultiplier 750V.

Chromatogram provided by Nieves Sarrión de KONIK-TECH, S.A

Peak Name 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38

a-Pinene Camphene β-Pinene 3-Carene β-Myrcene Limonene Eucalyptol g-Terpinene cis-β-Ocymene p-Cymene Terpinolene Methyl octanoate (-)-Camphor Linalool Linalyl acetate Methyl decanoate 4-Terpineol Linalyl isobutyrate (-)-Menthol Citronellol acetate Terpineol acetate (-)-Borneol a-Terpineol Nerol acetate Geraniol acetate Citronellyl Nerol β-Phenethyl Estragole Geraniol Hidroxycitronellal 1-Undecanol Cinnamal Eugenol Nerolin Thymol Carvacrol DEP

TKG 1228

SEPARATION OF METHYL ESTERS (FAMES) Column: TR-CN100, P/N TR-882192 Size: 100m x 0.25mm x 0.20µm Injection: 1µL Total FAMES en CH2Cl2 (30 mg/mL), split 1:100, 260ºC Carrier gas: He 45 psi, 21 cm/s (140ºC) Program temperature: 140ºC(6min) @ 4ºC/min to 240ºC(10min) Detector: FID, 260ºC Peak Name 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

C4:0 (butyric) C6:0 (caproic) C8:0 (caprylic) C10:0 (capric) C11:0 (undecanoic) C12:0 (lauric) C13:0 (tridecanoic) C14:0 (myristic) C14:1 (myristoleic) C15:0 (pentadecanoic) C15:1 (cis-10-pentadecanoic) C16:0 (palmitic) C16:1 (palmitoleic) C17:0 (heptadecanoic) C17:1 (cis-10-heptadecenoic) C18:0 (stearic) C18:1n9t (elaidic) C18:1n9c (oleic) C18:2n6t (linolelaidic)

20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37

C18:2n6c (linoleic) C20:0 (arachidic) C18:3n6 (g-linolenic) C20:1n9 (cis-11-eicosenoic) C18:3n3 (a-linolenic) C21:0 (henicosanoic) C20:2 (cis-11,14-eicosadienoic) C22:0 (behenic) C20:3n6 (cis-8,11,14-eicosatrienoic) C22:1n9 (erucic) C20:3n3 (cis-11,14,17-eicosatrienoic) C20:4n6 (arachidonic) C23:0 (tricosanoic) C22:2 (cis-13,16-docosadienoic) C24:0 (lignoceric) C20:5n3 (cis-5,8,11,14,17-eicosapentaenoic) C24:1 (nervonic) C22:6n3 (cis-4,7,10,13,16,19-docosahexaenoic)

TKG 1229 386

Foods & Flavors Peak Name 1 123456789101112131415161718192021222324252627282930313233343536-

C6:0 C4:0 (butyric) C6:0 (caproic) C8:0 (caprylic) C10:0 (capric) C11:0 (undecanoic) C12:0 (lauric) C13:0 (tridecanoic) C14:0 (myristic) C14:1 (myristoleic) C15:0 (pentadecanoic) C15:1 (cis-10-pentadecanoic) C16:0 (palmitic) C16:1 (palmitoleic) C17:0 (heptadecanoic) C17:1 (cis-10-heptadecenoic) C18:0 (stearic) C18:1n9c (oleic) + C18:1n9t (elaidic) C18:2n6c (linoleic) C18:2n6t (linolelaidic) C18:3n6 (g-linolenic) C18:3n3 (a-linolenic) C20:0 (arachidic) C20:1n9 (cis-11-eicosenoic) C20:2 (cis-11,14-eicosadienoic) C20:3n6 (cis-8,11,14-eicosatrienoic) C21:0 (henicosanoic) C20:3n3 (cis-11,14,17-eicosatrienoic) C20:4n6 (arachidonic) C20:5n3 (cis-5,8,11,14,17-eicosapentaenoic) C22:0 (behenic) C22:1n9 (erucic) C22:2 (cis-13,16-docosadienoic) C23:0 (tricosanoic) C24:0 (lignoceric) C22:6n3 (cis-4,7,10,13,16,19-docosahexaenoic) C24:1n9 (nervonic)

SEPARATION OF FAMES 1 2 12 3

Column: SupraWax-280, P/N TR-830232 Dimensions: 30m x 0.25mm x 0.25µm Injection: 1µL SP-47885-U (10 mg/mL), split 1:100, 260ºC Carrier gas: He, 30 psi (206.7 KPa) Oven program: 50ºC(2min) @ 4ºC/min to 220ºC(15min) Detector: FID, 260ºC

4 6

8 17,18 16

5

23

7 9 11 10

31 13 15 19 20 21 24 22 25 30 26 14 28 27 29 32 33 35

36

34

37

TKG 1237

SEPARATION OF METHYL ESTERS IN SOYA OIL Column: TR-CN100, P/N TR-882162 Size: 60m x 0.25mm x 0.20µm Injection: 1µL Total FAMES en CH2Cl2 (30 mg/mL), split 1:100, 280ºC Carrier gas: He 25 psi Program temperature: 185ºC Detector: FID, 280ºC

Peak Name 1 2 3 4 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22

C6:0 C8:0 C10:0 C12:0 C14:0 C15:0 C16:0 C16:1 C18:0 C18:1t C18:1 C18:2t C18:2t C18:2 C20:0 C18:3t C18:3 C20:1 C20:2 C22:0 C22:1

TKG 1239 387

Foods & Flavors GLYCOLS IN WINE Column: SupraWAX-280, P/N TR-831035 Size: 30m x 0.53mm x 1.0µm Injection: 1 µL split 1:50, 220ºC Carrier gas: He, 5.4 psi Program temperature: 100ºC (3min) @ 4.5ºC/min to 220ºC (10min) Detector: FID, 260ºC

RT (min) 13.20 14.20 23.16

Peak Name Propylene glycol Ethylene glycol Diethylene glycol

Chromatogram provided by R. Franquet and Joan Garcia from INCAVI (Vilafranca del Penedés, Barcelona)

TKG 1241

METHANOL AND HIGHER ALCOHOLS Column: TRB-624, P/N TR-601432 Size: 30m x 0.25mm x 1.4µm Injection: 1 µL split 1:50, 250ºC Carrier gas: He, 1mL/min Program temperature: 40ºC (5min) @ 20ºC/min to 200ºC (3min) Detector: FID, 260ºC

RT (min) 3.21 4.66 7.29 8.16 8.86 9.60 10.87 10.92

Peak Name Methanol Ethanol 1-Propanol 2-Butanol Isobutanol 1-Butanol Isoamylic alcohol Isoamylic alcohol

Chromatogram provided by Joan Garcia from INCAVI (Vilafranca del Penedés, Barcelona)

TKG 1242 388

Foods & Flavors PUFA I Column: SupraWax-280, 15m x 0.10mm x 0.10µm (P/N: TR-830111) Injection: 280ºC, split 200:1, precission liner Detector: FID, 280ºC Carrier Gas: H2, 45 psi (310.05 KPa) Oven: 100ºC (0.5min) @ 50ºC/min to 280ºC (2min) Sample: 0.2µL PUFA I - Marine Source diluted to 50mg/ml in methylene chloride Peak Name 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16.

C14:0 C16:0 C16:1v7 C18:1v9 C18:1v7 C18:2v6 C18:3v3 C18:4v3 C20:1v9 C20:1v11 C20:4v3 C20:5v3 C22:1v11 C22:1v9 C22:5v3 C22:6v3

TKG 1248

PUFA II Column: SupraWax-280, 15m x 0.10mm x 0.10µm (P/N: TR-830111) Injection: 280ºC, split 200:1, precission liner Detector: FID, 280ºC Carrier Gas: H2, 45 psi (310.05 KPa) Oven: 100ºC (0.5min) @ 50ºC/min to 280ºC (2min) Sample: 0.2µL PUFA II - Animal Source diluted to 50mg/ml in methylene chloride

Peak Name 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18.

C14:0 C16:0 C16:1v7 C18:0 C18:1v9 C18:1v7 C18:2v6 C18:3v6 C18:3v3 C20:1v9 C20:2v6 C20:3v6 C20:4v6 C20:5v3 C22:1v9 C22:4v6 C22:5v3 C24:1

TKG 1249 389

Foods & Flavors PUFA III Column: SupraWax-280, 15m x 0.10mm x 0.10µm (P/N: TR-830111) Injection: 280ºC, split 200:1, precission liner Detector: FID, 280ºC Carrier Gas: H2, 45 psi Oven: 100ºC (0.5min) to 280ºC @ 50ºC/min (2min) Sample: 0.1µL PUFA III – Partially Hydrogenate Menhaden Oil diluted to 100mg/ml in hexane

Peak Name 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19.

C14:0 C16:0 C16:1v7 C16:2v4 C16:3v4 C16:4v1 C18:0 C18:1v9 C18:1v7 C18:2v6 C18:3v4 C18:3v3 C18:4v3 C20:1v9 C20:4v6 C20:4v3 C20:5v3 C22:5v3 C22:6v3

TKG 1253

37 FAME MIX

Peak Name

Column: TR-CN100, P/N TR-881674 Size: 75m x 0.18mm x 0.14µm Injection: 1µL 37 FAME MIX (47885-U), split 1:100, 250ºC Carrier gas: H2 40 cm/s (140ºC) Program temperature: 140ºC(5min) @ 4ºC/min to 240ºC(2min) Detector: FID, 250ºC

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18

C4:0 C6:0 C8:0 C10:0 C11:0 C12:0 C13:0 C14:0 C14:1(cis-9) C15:0 C15:1(cis-10) C16:0 C16:1(cis-9) C17:0 C17:1(cis-10) C18:0 C18:1(trans-9) C18:1(cis-9)

19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37

C18:2(all-trans-9,12) C18:2(all-cis-9,12) C18:3(all-cis-6,9,12) C18:3(all-cis-9,12,15) C20:0 C20:1(cis-11) C20:2(all-cis-11,14) C20:3(all-cis-8,11,14) C20:3(all-cis-11,14,17) C20:4(all-cis-5,8,11,14) C20:5(all-cis-5,8,11,14,17) C21:0 C22:0 C22:1(cis-13) C22:2(all-cis-13,16) C22:6 (all-cis-4,7,10,13,16,19) C23:0 C24:0 C24:1(cis-15)

TKG 1250 390

Foods & Flavors 37 FAME MIX- MS DETECTOR Column: TR-CN100, 60m x 0.25mm x 0.20µm (P/N: TR-882162) Injection: 280ºC, split 50:1 Oven: 90ºC (7min) to 240ºC @ 4ºC/min (3min) Carrier gas: Helium, constant pressure @ 24psi Detector: MS Transfer line temp.: 230ºC Ionization mode: EI Scan range: 40-450amu Sample: 0.5µL Food Industry FAME Mix 30mg/ml in methylene chloride Peak Name 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18

C4:0 C6:0 C8:0 C10:0 C11:0 C12:0 C13:0 C14:0 C14:1(cis-9) C15:0 C15:1(cis-10) C16:0 C16:1(cis-9) C17:0 C17:1(cis-10) C18:0 C18:1(trans-9) C18:1(cis-9)

19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37

C18:2(all-trans-9,12) C18:2(all-cis-9,12) C18:3(all-cis-6,9,12) C18:3(all-cis-9,12,15) C20:0 C20:1(cis-11) C20:2(all-cis-11,14) C20:3(all-cis-8,11,14) C20:3(all-cis-11,14,17) C20:4(all-cis-5,8,11,14) C20:5(all-cis-5,8,11,14,17) C21:0 C22:0 C22:1(cis-13) C22:2(all-cis-13,16) C22:6 (all-cis-4,7,10,13,16,19) C23:0 C24:0 C24:1(cis-15)

TKG 1251 391