Decontamination of tetramethylammonium hydroxide (TMAH

Laramie, Wyoming, USA, 3Colorado School of Public Health, Denver ... Tetramethylammonium hydroxide (TMAH), used in microelectronic industries and rese...

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Cutaneous and Ocular Toxicology, 2010, 1–6, Early Online

RESEARCH ARTICLE

Cutaneous and Ocular Toxicology Downloaded from informahealthcare.com by Dr Laurence Mathieu For personal use only.

Decontamination of tetramethylammonium hydroxide (TMAH) splashes: promising results with Diphoterine® in vitro Céline Fosse1, Laurence Mathieu1, Alan H. Hall2,3, Elena Bocchietto4, François Burgher1, Michel Fischbach4, and Howard I. Maibach5 Laboratoire Prevor, Valmondois, France, 2Toxicology Consulting and Medical Translating Services, Inc. (TCMTS, Inc.), Laramie, Wyoming, USA, 3Colorado School of Public Health, Denver, Colorado, USA, 4ABICH Srl, Verbano, Italy, and 5 Department of Dermatology, University of California–San Francisco, San Francisco, California, USA 1

Abstract Tetramethylammonium hydroxide (TMAH), used in microelectronic industries and research and development, has both corrosive properties and systemic toxicity. Two fatal TMAH occupational exposure cases have been published. Studies comparing initial TMAH decontamination with Diphoterine® versus tap water were performed: an in vitro pH titration study and an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) in vitro cytotoxicity cell viability assay. For pH normalization, 17 times more tap water than Diphoterine® was required. In the cytotoxicity test, two-thirds of the cells remained viable after Diphoterine® washing, compared with only one-third after tap water washing (p